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ROS Detection Assay Kit
The ROS Detection Assay kit (Green Fluorescence) is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA).
Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidized by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.
For quantitative measurement of cellular Reactive Oxygen Species (ROS) in cells
Main Features
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Measure ROS production in live cells
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Ideal for fluorescence microscopy, microplate titration and cytometry
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Compatible with adherent cells as well as suspension cells
Kit content
For up to 300 assays in 96-well plate- 25mM DFC-DA in DMF (35µL) - 10X ROS Buffer (5 mL)
- Tert-Butyl Hydrogen Peroxide (TBHP) 25 mM (50 µL)
Each ROS Kit provides sufficient reagents for approximately 300 measurements in microplate format and includes Tert-Butyl hydroperoxide (organic peroxide) solution provided as a positive control for ROS generation.
Storage: Upon reception, store the DFC-DA tube (avoid multiple freeze/thawing cycles) and the 10X ROS Buffer at -20°C. Store the TBHP tube at 4°C.
Shipping Conditions: Shipped with ice pack
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Catalog number | Unit Size | Price excl tax | Quantity / Buy |
ROS0300 |
300 Assays
|
$267.00
|
|
Citation database
Download
ROS Assay kit rapid protocol
Download 588.72KBROS Assay Kit MSDS
Download 408.37KBAssay Kits Brochure
Download 612.8KBRelated products