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ROS Detection Assay Kit
  • ROS Detection Assay Kit
  • ROS Detection Assay Kit

ROS Detection Assay Kit

The ROS Detection Assay kit (Green Fluorescence) is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA).

Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidized by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.  

For quantitative measurement of cellular Reactive Oxygen Species (ROS) in cells

Main Features

  • Measure ROS production in live cells

  • Ideal for fluorescence microscopy, microplate titration and cytometry

  • Compatible with adherent cells as well as suspension cells

 

Kit content

For up to 300 assays in 96-well plate
  • 25mM DFC-DA in DMF (35µL) - 10X ROS Buffer (5 mL)
  • Tert-Butyl Hydrogen Peroxide (TBHP) 25 mM (50 µL)

Each ROS Kit provides sufficient reagents for approximately 300 measurements in microplate format and includes Tert-Butyl hydroperoxide (organic peroxide) solution provided as a positive control for ROS generation. 

Storage: Upon reception, store the DFC-DA tube (avoid multiple freeze/thawing cycles) and the 10X ROS Buffer at -20°C. Store the TBHP tube at 4°C.

Shipping Conditions: Shipped with ice pack

 

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Catalog number Unit Size Price excl tax Quantity / Buy
ROS0300
300 Assays
$267.00

Citation database

1321N1
13762 Mat B III
143B
15P-1
16HBE
184BJ
208F
2PK3
32Dcl3
3A6
3T3
3T3-J2
3T3-L1
3T6
3Y1
44S
4T1
5637
59M
661W
786-O
804G
8505C
9HTE
A10
A1207
A172
A20
A204
A2058
A2780
A2780/AD
A375
A431
A498
A549
A6
A673
A72
A7r5
A875
AB1
Abdominal cavity
AC16
ACHN
Acinar Cells
Adherent gastric cells
Adipocytes
AdSC
AE-1
AFSC
AKR
All cells
AML
Amnion cells
Amphophoenix cells
Anjou 65
Anterior pituitary gonadotropes
Aortic root
AR4-2J
ARPE-19
Astrocyte derived cells
Astrocytes
Astrocytesﾠ: neural stem cell derived
ATDC5
AtT-20
AU-565
Axons
B Lymphocytes
B-TC3
B104
B11
B16-F1
B16-F10
B35
B6
B65
B82
B95a
Ba/F3
BAC
BAEC
BC3H1
BCEC-1
BE(2)-C
BE(2)-M17
Beas-2B
BEC
BEL-7402
BeWo
BFC012
BHK-21
BIU-87
BJ5ta
BJAB
BLCL
BLM
Bm5
BME6
BMEC – Brain microvascular endothelial cells
BMSC
BNL CL.2
Bone marrow cells
Bone Marrow derived macrophages
Bone marrow derived macrophages – BMDM
Bone marrow derived stromal cells
bone marrow monocytes
Bone marrow mononuclear cells
Bone Marrow Progenitor
Bowes melanoma cells
Brain
BRCA cell lines
Breast cancer cell lines
BRL-AG-3C
Bronchial epithelial cells
BSC-1
BSC-40
BSM
BSR-T7
BT-20
BT-474
BT-477
BT-549
BTK143
BV-2
C-33A
C127
C13-NJ
C1R
C20
C272
C2C12
C3H 10T1/2
C57MG
C6
C6/36
CaCo-2
CAK
CAL-1
Cal120
Cal27
Cal51
Calu-1
Calu-3
Calu-6
CAMA-1
CAP Spheroid
Capan-2
Cardiac progenitor
Cardic cells
Cardiomyocytes
Cardiomyocytes cell line
Carotid artery smooth muscle
CaSki
CATH.a
CCD-1064sk
CD34+
CEF
CEM
CEMx174
Cf2Th
CFK2
CG-4
CHLA-15
CHLA-20
CHO
CHO-IG1
CHO-IG3
CHO-K1
CHO: suspension
Chondrocytes
Chromaffin cells
CHU-2
CJ7
CL7.1
Cl8
CLU-301
CMT-93
COH
Colo205
Colon cancer cells
Colonic endothelial cells
COS-1
COS-7
COV362
CP70
cPTC
CRFK
CS-1
CT26
CT51
CT60
CTLL-2
CV-1
CWR22Rv1
D3
D3H2LN
D660
Daoy
DAUDI
DC2.4
Dendritic cells
DF1
Distal convulated tubules
DLD-1
DRG cells
DT40
DU145
Ductal adenocarcinoma
E14TG2a
EA.hy926
Ear artery
EBC-1
ECC-1
ECV-304
EL-4
Embryonic stem cells
EndoC beta H1
Endometrial stromal cells
Endothelial cells
Endothelial cells : Aorta
Endothelial colony forming
Endothelial Progenitors
Epicardial Mesothelial cells
Epithelial cells
Epithelial Lung cells
Epithelial organoid
EPP85-181
ER-Hoxb8
ES Embryonic Stem
ES-2
ES-D3
Ex vivo: AGM
Ex vivo: Aorta
Ex vivo: Brain Slice
Ex vivo: Kidney cryosection
Ex vivo: Retinal Explant
Ex vivo: retro-orbital injection
Ex vivo: Tendon
Ex vivo: Urogenital ridges
Ex vivo : pancreatic section
Ex vivo : porcine retinal explant
Ex vivo : porcine whole eye
Expi293
F442-A
F9
FaDu
FGC-4
Fibroblast cell line
Fibroblast like synoviocytes
Fibroblasts
Fibrochondrocytes
Fibroid Stem Cells
Fibrosarcoma
FL5.12
FM3A
Foetal neurons
Foreskin Fibroblast
FR
FSDC
FTO-2B
G401
G402
Gastric gland
Gastric myofibroblasts
Gastric Parietal Cells
GC-2spd (ts)
GD25
GDTC
GH3
GH4C1
GL261
Glioblastoma
Glioma cells
Glioma Stem cells
Granta-519
Granulosa cells
GT1
GTL-16
H-500
H1
H1299
H13
H146
H1792
H187
H19-7
H1994
H226
H23
H292
H295R
H35
H4
H441
H4IIE
H661
H7
H9
H9c2
HA847
HaCaT
HAEC
HAPI
HB60
HBEC
HBL-100
HBMEC
HBTE
HC11
HCA-7
HCAEC
hCBMC
HCC-1806
HCC-1954
HCC-38
HCC-4006
HCEn
HCT116
HCT15
HCT8
HD180
HDF
HEC-1-A
HEC-1-B
HEK-293
HEK-293: suspension
HEK-293A
HEK-293F
HEK-293T
HEL
HeLa
Hematopoietic Stem Cells
Hep2
Hep3B
Hepa 1-6
Hepatic Stellate Cells
hepatocellular carcinoma
Hepatocytes
HepG2
HepG3
hESC
HFF
HFF1
HFFF2
HiB5
High 5
hiPSC
HIT-T15
HITB5
HK2
hKC
HL-1
HL-5
HL-60
HMC3
HMCB
HMEC
HMEC-1
HMy2.C1R
HN12
HNP1
HNSCC
HOS
HOSE
HPAEC
HPE
HRE
Hs 578T
HS-578
HS-729T
Hs68
HSC
HSC-3
HSC39
HSC43
HSC45
HSC57
HSC59
HSG
HSPC
HSY-EB
HT1080
HT22
HT29
HTLA230
HUC-HSC
HUC-MSC
Huh-7
Human Aortic Smooth Muscle Cells
Human dermal fibroblast
human Embryonic Stem Cells
Hut 78
Hut-102
HuTu 80
HUVEC
IB3-1
IC-21
IDP
IE-6
IEC-6
IGR-39
IGROV-1
Immature HSC
IMR-32
IMR-90
in vivo
in vivo Jejunum
in vivo Stomach
in vivo: Abdominal cavity
in vivo: Aortic root
in vivo: Brain
in vivo: Calvarial defect
in vivo: Ear artery
in vivo: Endothelial cells
in vivo: Fibrosarcoma
in vivo: hepatocellular carcinoma
in vivo: intramuscular injection
in vivo: intratechal injection
in vivo: left adductor muscle
in vivo: Lung
in vivo: Peri anal region
in vivo: Pupae
in vivo: sciatic nerve
in vivo: Skin Flap Model
in vivo: subarachnoid space
in vivo: subcutaneous injection
in vivo: Subcutaneous tumor
in vivo: Trypanosoma cruzi
in vivo : brain
in vivo : ear
in vivo : eye topical & IVT
in vivo : hippocampus
in vivo : inguinal adipose tissue
in vivo : intra cerebellar
in vivo : intradermal
in vivo : intraperitoneal
in vivo : intratumoral
in vivo : intravenous injection
in vivo : superficial temporal vein
INS-1
Intestinal Spheroids
IPC-298
iPS
IVEC
J558/L
J774
J82
JB6
JEG-3
Jejunum
JIMT-1
Jurkat
JY
K46
K562
KB
Kc
KC18-2-40
KD83
Kelly
Keratinocytes
KG-1a
KGN
Kidney Ascending Limb cells
Kidney section
KM12
KMM-1
KMS-11
KMS-12
KMS-5
KMST-6
KNRK
KPC
KPON
KS
KS-1
KT-3
L-Cells
L-M(TK-)
L02
L1210
L2
L6
L6E9
L8
L929
LAN-5
LEC
left adductor muscle
Liver cells
LK35.2
LLC
LLC-PK1
LLE/LULS45
LMH
LN308
LN428
LNCap
LNT-229
LoVo
LS 174T
LS180
LSK / LKS
LSV5
LUHMES
LULS45
Lung
Lymphocytes
Lymphoid (Oka)
M-1
M1
M21
M21-L4
MA-10
Macrophages
Magi-CCR5
MC38
MC3T3-E1
Mca
McA-RH7777
MCF-10A
MCF-10DCIS
MCF-12A
MCF-7
MCF7
MDA-MB-231
MDA-MB-435
MDA-MB-436
MDA-MB-453
MDA-MB-468
MDA-MB-549
MDAH-2274
MDBK
MDCK
MDST8
ME-180
Medaka Cells
MEF
MEL
Melanocytes
Melanoma cells
Meningioma cells
mES
MES-SA
MESC2.10
Mesencephalic Cells
Mesenchymal Stem Cells
MeWo
MEXF-2090
Mf4/4
MG-63
MGC-803
MIA PaCa-2
Microglial
mIMCD
mIMCD-3
MIN6
Mitochondrial xenocybrid
MKN-28
MKN-45
MKN-7
MLE15
MLEC32
mlEnd
MM1S
MM6
MN9D
MNNG/HOS
MNT1
MoB
MOLT-4
Monocytes
Mononuclear cells
mouse Embryonic Stem Cells
MRC-5
MS-5
MS1
MSC
MT2
MT4
MTD-1A
MTLn3
MTSV1-7
Mutu I
Mutu III
Mv 1 Lu
Myeloid progenitor
Myoblasts
Myocytes
Myofibroblasts
Myometrial
N13
N1E-115
N2a
N87
N9
NALM-6
NB324K
NB4
NBT-II
NCB-20
NCB-21
NCB-22
NCB-23
NCB-24
NCB-25
NCB-26
NCB-27
NCB-28
NCB-30
NCB-31
NCB-34
NCB-35
NCB-37
NCB-39
NCB-40
NCB-41
NCB-42
NCI-H2228
NCI-H23
NCI-H292
NCI-H295
NCI-H295R
NCI-H358
NCI-H441
NCI-H460
Neural Crest Cells
Neural progenitor cells
Neural Stem Cells
Neuro-2a / N2a
Neuroblastoma
Neuroblasts
Neurons
Neurons: amygdal
Neurons: Basal ganglia
Neurons: cerebellar granule (CGN)
Neurons: cervical ganglion
Neurons: cortical
Neurons: cortical embryonic DRG
Neurons: cortical-IPS derived
Neurons: dopaminergic neurons
Neurons: dopaminergic neurons (iPSC derived)
Neurons: dorsal root ganglion
Neurons: embryonic cortical
Neurons: embryonic motor neuron
Neurons: excitatory
Neurons: excitatory – iPSC derived
Neurons: ganglia
Neurons: Hippocampal
Neurons: hypothalamic
Neurons: IPS derived from i3N
Neurons: motor neurons
Neurons: Motor neurons derived from ES cells
Neurons: Motor neurons derived from iPSC
Neurons: neocortical
Neurons: Neural Stem cells derived
Neurons: nodose ganglions
Neurons: septal
Neurons: Spinal cord
Neurons: Spiral Ganglion Neurons ﾖ SGN
Neurons: striatal
Neurons: superior cervical ganglia - SCG
Neurons: sympathetic
Neurons: vagal afferents
Neurons: ventral mesencephalons
NG108-15
NHBE - normal human bronchial/tracheal epithelial cells
NHDF - normal human dermal fibroblasts
NHF3
NHFF
NIH-3T3
NIH-3T6
NIH: OVCAR-3
NLSm cells
NMuMG
NRK - normal rat kidney epithelial cells
NS20Y
NSC19
NSC34
NTERA-2 cl.D1 (NT2/D1)
Nucleus pulposus
NYGM
O23
OK
Olfactory Mucosal cells
OLHNI-2
Oligodendrocyte Precursors
Oligodendrocytes: NSC derived
OLN-93
OPAEC
Orbital fibroblast
Organoids
OS3
OSE
Osteoblasts
OV-90
Ovarian cancer cell
Ovarian cells
OVCAR5
P19
P388D1
P3XAg8.653
P815
P825
PA-1
PA317
PAEC
Paju
Pam212
PANC-1
Pancreatic Islets
Pancreatic stelatte cells
Papilla cells
Parietal Cells
PASMC
PBL
PBMC
PC-12
PC-3
PC-9
PCI-13
PDAC
PER.C6
Pex5+/-
Phoenix -ampho / Phoenix-Eco cells
Pinealocytes
Plat-E
PLB-985
PNT2
Pollen
Pre-B
PrEC
Progenitor cells (pancreatic)
Proximal Tubule Epithelial Cells-PTEC
PT67
PU5-1.8
Pupae
QGY-7703
QT35
QT6
R1/E
rabbit pleural mesothelial
RAEC
Raji
Ramos (RA1)
Rat adipocyte
Rat epithelial cells
Rat tracheal epithelial cells
Rat1
Rat2
Rat6
RAW 264.7
RCHO / RCHO-1
RCME - rabbit coronay microvessel endothelial cells
RDB
Retinal ganglion cells
RIE-1
RIN 1046-38
RK13
RKO
RL95-2
ROS 17/2.8
RPE
RPE-1
RPE.40
RW-4
RWPE1
S194
S2
S2-CP10
S2-CP8
S2-CP9
S2/H
S2R+
S49.1
Saos-2
Satellite Cells
SC1 REW22
SCC-15
SCC-25
SCC-7
SCLC – small cell lung cancer
Sertoli Cell
sf21
sf21ae
sf9
SH-SY5Y
SHEP
SHP-77
SiHa
SIM-A9
SK-BR-3
SK-Hep-1
SK-LMS-1
SK-MEL-1
SK-MEL-28
SK-Mel-37
SK-N-AS
SK-N-BE(2)
SK-N-MC
SK-N-SH
SK-OV-3
SK-UT-1
Skin Flap Model
SL2
SM10
SMA-560
SMMC-7721
Smooth muscle cells
SNB-19
SNU-441
SNU-449
SolC1(-)
SOLD6
SP2/0-Ag14
Spermatozoa
Splenocytes
SPOC1
STC-1
Stomach
Striatal cells
Subcutaneous tumor
SUIT-2
SUP-T1
SV-MRC5
SVEC
SVEC-4
SVR
SW13
SW480
SW620
SW626
SW684
SW837
SW982
Synoviocytes
T lymphocytes
T lymphocytes CD4+
T lymphocytes CD8+
T-47D
T-Rex-293
T-Rex-CHO
T-Rex-HeLa
T24
T265
T3CHO/AT1A
T47D
T84
T98G
TA3/Ha
TDC Tendon derived cells
TE671
Tera-2
TGW-nu-1
Thick ascending limb cells
THP-1
Thymic Lymphoma
TIF
TIG-1
TIG-3
TIG3
TK-1
TKD2
TM4
TOV-112D
TOV-LT
Trabecular Meshwork
Tracheal Myocyte
Trypanosoma cruzi
TS/A
tsA201
TTF Tail Tendon Fascicels
TYK-nu
TZM-bl
U-118
U-118 MG
U-138 MG
U-2-OS
U-251
U-251 MG
U-373
U-87
U-87 MG
U-937
U266B1
UC 729-6
UMR 106
UMRC6
UOK 257
UOK 262
URO sa
Vero
Vero 10A1
Vero E6
VOT-E36
Vsa13
Vsa16
VSMC
W9.5
WEHI-231
WI-38
WM-1158
Wm-233-4
WM-239-A
WM-266-4
WM-852
XPC15
XPC16
Y-1
Y79
YH-13
YK6-1
Z-R75-1
ZR-75-1
0
3D Transfection DNA
3D Transfection siRNA
AAV
Adeno-Associated Virus (2)
Adenovirus
alphavirus
Antibody
brain extract
cmRNA
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flavivirus
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shRNA encoded plasmid
shRNA-encoding plasmid
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GFP mRNA
Glial-Mag
h IL-2 mRNA
h Sox2 mRNA
HA(-TMD)-H1N1 mRNA
HA(FL)-H1N1 mRNA
HA-H3N2 mRNA
HeLaFect
Helix-IN Transfection Reagent
HYPE-293
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HYVIR™ Transfection Reagent
IFAVax
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IVT Kit
LDH Cytotoxicity Assay Kit
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LentiBlast Premium Superior Grade
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LipoVax NTA(Ni)
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NanOZ LNP-DIY(105S)
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NanOZ LNP-DIY(SM102)
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NeuroMag
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ONPG kit
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OZBlue Cell Viability Kit
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saRNA GFP
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si3D-Fect
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SilenceMag
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ViroMICST
ViroMICST Stem Transduction Enhancer
WST-8 Cell Proliferation Kit
X-Gal Staining kit
X-Gal Substrate
XPMag
β-Gal mRNA

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ROS Assay kit rapid protocol

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