Custom mRNA Synthesis Service

You can easily request a quote by filling in our dedicated form available here. If you need further guidance or wish to discuss your project and specific requirements with our technical team, feel free to contact us — we will be happy to arrange a meeting at your convenience.

Custom mRNA synthesis takes approximately 2 to 6 weeks (depending on the source of the DNA template). For projects also requiring downstream formulation, such as LNP encapsulation, please refer to our dedicated LNP formulation service for associated timelines.

The minimum order quantity for custom mRNA synthesis is 200µg. There is no fixed upper limit: production is scaled to your needs. Please contact us to discuss larger quantities.

Yes. Codon optimization is available for the species of your choice, including human, mouse, and rat. Optimizing codon usage can significantly improve translation efficiency and protein yield in the target organism, and is particularly recommended for constructs intended for in vivo use or when high expression levels are required.

When ordering a custom mRNA from OZ Biosciences, you can either:

• give us the sequence of your gene and we handle the DNA synthesis,
• or send us your DNA template.

If you choose the second option, your DNA template needs to:

1. Be linearized (or contains a restriction site after the Poly(A) tail

2. Includes a T7 promoter and a Poly(A) tail sequence*

*The Poly(A) tail can be added after in vitro transcription as an additional service (if asbent from DNA template).

3. Be purified (methods of your choice, i.e.) and oberve ratio of A260/A280 and A260/A230 > 1.8

For 1mg of mRNA, we will require ~20µg of purified linear DNA.

For more details, please refer to our DNA plasmid requirement guideline.

Our standard constructs contain 5' and 3' untranslated regions (UTRs) selected to support mRNA stability and translation. You can also provide your own UTR sequences, or ask us to select UTRs suited to your application.

We typically produce mRNA from 250 to 12,000 nucleotides in length. Requests outside this range are also considered: please contact us with your sequence and we will assess feasibility.

The mRNA is supplied at 1 mg/mL ± 5 % in 1 mM sodium citrate buffer, pH 6.4. Other concentrations and buffers are available on request.

All our custom mRNAs are purified and undergo rigorous quality control before delivery. We offer two levels of QC depending on your requirements:

Standard QC includes:

• Concentration measurement (spectrophotometry).
• Purity assessment via 260/280 and 260/230 absorbance ratios.
• Integrity analysis by agarose gel electrophoresis and HPLC profiling.
• Sterility testing.

Superior Grade QC includes all Standard QC checks, plus:

• Endotoxin testing.
• dsRNA quantification.
• Protein expression (when possible, i.e. fluo proteins, antibody available.)

Please refer to our mRNA quoting tool to select the QC level that best fits your project requirements.

By default, 1 mg of mRNA is supplied in a single vial at 1mg/mL. However, aliquoting is available upon request. Simply specify your preferred aliquot size or number of vials when placing your order and we will accommodate your needs.