IVT Kit
This kit is designed for the in vitro transcription of your linear plasmid DNA or PCR fragment using the T7 polymerase with a yield of approximately 100 µg of RNA per µg of DNA template. It is provided with sufficient components for twenty-five reactions (20µl each). Each kit will yield a total of 2.5 mg of RNA. This corresponds to 6.7 nanomoles (25 reactions) of RNA based on the control template provided (1038 nt). The yield will highly depend on each DNA template, sequence, length, and purity.
NOTE:
- Shorter templates typically yield a lower mass and a higher molar yield of RNA product.
- The expected yield of 100µg is achieved with the use of our DNA control template (leading to a 1038 nucleotides IVT product) and non-modified nucleotides. Every modification of the protocol, for example the use of chemically modified nucleotides or Cap analogs can affect the reaction and lead to a diminished yield.
The RNA generated using this kit can be employed in a diverse range of experiments such as in vitro translation, cell transfection, isolation of RNA binding proteins, etc...
The kit is provided with sufficient reagents to perform 25 in vitro transcription reactions.
Storage: Upon receipt and for long-term use, store reagents at the recommended storage conditions (see table above). Kit’s components are stable for at least 1 year
Shipping conditions: Transcription kit is shipped with dry ice.
Kit contents:
- pDNA template Control 1mg/ml
- T7 Enzyme mix, 50µl
- ATP (75mM), 50µl
- GTP (75mM), 50µl
- CTP (75mM), 50µl
- UTP (75mM), 50µl
- IVT 10X Buffer, 50µl DNase 1U/µl, 25µl
- Nuclease free water, 1ml
- Precipitation solution LiCl 7.5M, EDTA 0.5M pH8, 750µl
- Gel loading dye 2x 47.5% Formamide, 0.5 mM EDTA, 0.01% SDS, 0.01% SDS, 0.01% Bromophenol Blue, 0.005% Xylene Cyanol), 1ml
Material not provided with the kit :
- 70% ethanol for RNA washing.
- Nuclease-free buffer (1mM citrate buffer pH 6.4, TE buffer or RNAse-free water) for RNA resuspension.
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| IVT-K25 |
25 reactions
|
$375.00
|
|
IVT-K25
25 reactions
$375.00
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