

Optimized Cas9 Nuclease S. Pyogenes is designed for genome editing in living cells or organisms and also for in vitro digestion.
Increased genome editing efficiency using Cas9/RNP delivery
Successful CRISPR/Cas9 genome editing can be performed through diverse approaches (plasmids, mRNA, nuclease, viral delivery)
Why choose Cas9 Protein instead of Cas9 DNA or mRNA?
The Cas 9 recombinant protein is delivered more rapidly than nucleic acid and is fully active once inside the cells without latency period (in contrast to transcription and translation machineries required for the nucleic acids).
These features make nuclease protein delivery particularly well suited for precision genome engineering.
Efficient nucleic acid delivery represents a critical step for genome editing experiments. For the most efficient Cas9 nuclease delivery, we recommend Pro-DeliverIN CRISPR Transfection Reagent.
Sizes
Reagent supplied: Cas9 reaction buffer (10X)
Special CRISPR/Cas9 Delivery Kit
Storage: Cas9 nuclease & Cas9 reaction buffer must be stored at -20°C. Avoid freeze/thawing cycles.
Shipping condition: Dry Ice
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Catalog number | Unit Size | Price excl tax | Quantity / Buy |
CAS9050 |
50 µg Cas9 nuclease
|
$126.00
|
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CAS9100 |
100 µg Cas9 nuclease
|
$223.00
|
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CAS9500 |
500 µg Cas9 nuclease
|
$907.00
|
|
CAS9PIC |
50 µg Cas9 nuclease + 100µL of ProDeliverIN CRISPR
|
$282.00
|
|
RECOMMENDED for CRISPR Cas9 Genome Editing experiments.
Optimized Cas9 Nuclease S. Pyogenes is designed for genome editing in living cells or organisms and also for in vitro digestion.
Genome editing in cells using Pro-DeliverIN CRISPR
Cas9 nuclease can be delivered directly into living cells in culture or organisms using ProDeliverIN CRISPR (# PIC60100 or #PIC60500). The protocol below is an example for genome editing in a 24-well plate:
For more information, please refer to ProDeliverIN CRISPR protocol or send an email to: tech@ozbiosciences.com
Cas9 nuclease derived from Streptococcus pyogenes. Contains a N-Terminal His Tag, 2 Optimized Nuclear Localization Sequences (NLS) – 1 N-terminal NLS and 1 C-terminal NLS + 1 genuine Targeting Sequence.
The Cas9 nuclease (CRISPR associated protein9) is a RNA-guided endonuclease used for genome editing by generating sequence-specific double stranded breaks (DSB). The presence of DSB in DNA leads to activation of cellular repair processes in which DNA restauration occurs through non-homologous end-joining (NHEJ) or homology-directed recombination (HDR) and thus to modification of genome. Gene knockdowns, deletions or insertions, illustrates the major achievement of the CRISPR/Cas9 system.
Fig1: The Cas9 nuclease programmed with sgRNA. Upon binding, the short guide RNA (sgRNA) specifically targets a short DNA sequence-tag (PAM). Cas9 nuclease cleaves DNA three nucleotides upstream the PAM sequence.
Fig2: Cas9 nuclease S. Pyogenes is used to cleave pVectOZ-GFP plasmid in vitro. pVectOZ-GFP was linearized using xhoI restriction enzyme and DNA was incubated in presence of Cas9 + sgRNA targeting GFP, Cas9 alone or Cas9 + sgRNA scramble (sc). In presence of targeting sgRNA, linearized DNA is cleaved in two fragments.
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