CPRG kit
OZ Biosciences CPRG assay kit is ideal to quantify low expression level of beta-Galactosidase. LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, b-Gal), is very stable, resistant to proteolytic degradation and easily assayed.
The levels of active beta-Gal actosidase expression can be quickly measured by its catalytic hydrolysis of CPRG (Chlorophenol red-b-D-galactopyranoside), substrate to a dark red product. The high sensitivity of this substrate improves the measurement of beta-Galactosidase activity when the reporter gene expression is low. (Absorbance at 570-595 nm).
All the necessary reagents are provided in this assay kit and offer a rapid, simple and sensitive method to quantify spectrophotometrically the enzyme expression level in LacZ transfected cells.
- Convenient for all transfection assays
- Suitable for cultured cells and tissues
- Simple, rapid and easy-to-use
- Economical
- Optimized with stable buffers
Kit contains: Lysis Buffer 55 mL, Standard Dillution Buffer 55 mL, 10X CPRG 5 mL, Substrate Buffer 55 mL, Stop Buffer 55 mL, Beta Gal Enzyme 100 µL
Storage: All reagents at +4°C except Beta Gal Enzyme and CPRG Substrate stock solution.Shipping Conditions: Gel pack Please log in or register to see the prices for your country
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| GC10002 |
All reagents
|
$281.00
|
|
GC10002
All reagents
$281.00
Citation database
| NAME | MOLECULE VECTOR | PRODUCT | REFERENCE | |
| all cells | 0 | CPRG kit | Ajuebor MN. J Virol. 2008 Oct;82(19):9564-76. | |
| all cells | 0 | CPRG kit | Buller CJ. Methods Find Exp Clin Pharmacol. 2003 Nov;25(9):713-6. | |
| all cells | 0 | CPRG kit | Dublin Institute of Technology 2010 | |
| all cells | 0 | CPRG kit | Eustice DC. Biotechniques. 1991 Dec;11(6):739-40 742-3. | |
| all cells | 0 | CPRG kit | J Biol Chem. 1994 Jan 28;269(4):2550-61 | |
| all cells | 0 | CPRG kit | Lim K. Biotechniques. 1989 Jun;7(6):576-9. | |
| all cells | 0 | CPRG kit | Pelisek J. J Vasc Res. 2000 Nov-Dec;37(6):585-93. | |
| all cells | 0 | CPRG kit | Petitdemange C. Clin Infect Dis. 2013 Sep;57(5):745-55. | |
| all cells | 0 | CPRG kit | Sahmi F. Theriogenology. 2012 Sep 1;78(4):724-30. | |
| all cells | 0 | CPRG kit | Seeber F. Gene. 1996 Feb 22;169(1):39-45. | |
| all cells | 0 | CPRG kit | USPATENT - US 2014/0037666 A1 | |
| all cells | 0 | CPRG kit | Zelphati O. Gene Ther. 1998 Sep;5(9):1272-82. |
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