SEAP Assay kit
SEAP Assay Kit (Secreted Alkaline Phosphatase) is a colorimetric assay for sensitive quantification of SEAP in culture medium from transfected cells or tissues. Alkaline phosphatase cleaves the colourless 4-nitrophenylphosphate (pNPP) substrate to give a yellow p-nitrophenolate product in alkaline solutions. It is commonly used as a reporter gene expression and can be accurately quantified. It is a truncated form of placental alkaline phosphatase (GPI-anchored protein).
The kit contains sufficient reagents to perform 500 assays in 96-well plate format.
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Designed to detect SEAP reporter gene expression
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Fast and easy analysis
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Colorimetric – measure with a standard spectrophotometer or an ELISA reader (405 nm)
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Multiple samples can be analyzed in low volumes
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Convenient and economical packaging: no tablet, no powder
Sizes:
pNPP-containing Assay Buffer component A: 10 x 1 mL
Reaction Buffer #1 component B: 125 mL
Reaction Buffer #2 component C: 125 µL
Dilution Buffer component D: 20 mL
Storage: -20°C for component A / +4°C for components B, C and D
Shipping Conditions: Room Temperature
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Catalog number | Unit Size | Price excl tax | Quantity / Buy |
SP00500 |
All reagents
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$176.00
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Citation database
NAME | MOLECULE VECTOR | PRODUCT | REFERENCE | |
all cells | 0 | Seap Assay kit | Pajarskienė J. Int Immunopharmacol. 2021 Oct 20;101(Pt A):108262. | |
all cells | 0 | Seap Assay kit | Čebatariūnienė A. Stem Cells Dev. 2019 Apr 24. doi: 10.1089/scd.2019.0021. | |
all cells | 0 | Seap Assay kit | Jonavičė U. J Tissue Eng Regen Med. 2019 Jan 16. doi: 10.1002/term.2810. |
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