OZ Biosciences offers solutions to reach optimal protein, antibody and virus production yield.
HYPE Transfection Kits: The go-to Bioproduction Solution
As the development of stably-expressing cell lines can be laborious and challenging, transient transfection in mammalian cells has become the go-to method for obtaining milligram to gram quantities of recombinant proteins in a matter of days.
A key to a successful TGE (Transient Gene Expression) system and efficient protein production is to use a specific and appropriate transfection reagent.
At OZ Biosciences, we have developed two alternative and efficient transfection kits, specific to HEK293 and CHO cells, the two widely used hosts for TGE.
Reach optimal Protein & Antibody Production yield
Hype-293
HYPE-293™ Transfection Kit is dedicated to achieve High Yield Protein Expression in HEK-293, 293-S, 293-F or any 293-related cells growing in suspension. Scale-up to larger volumes for production of protein per liter of cell culture is straightforward and easy with simple and cost efficient handling steps.
Main caracteristics:
- Reach optimal Protein & Antibody production yield.
- Suitable for all HEK293 cells growing in suspension.
- Compatible withany synthetic or regular media used for protein production.
- Ideal for bioreactor, spinner or flasks.
- Animal origin free.
HYPE-293™ Transfection Kit achieves the highest protein yield in comparison to other commercially available transfection reagents.
Figure 1: SEAP Expression with HYPE-293 Kit vs. other reagents. SEAP was produced by transient transfection using HYPE-293 Transfection Kit, Free Style Max transfection reagent, LP2K, FHD, 293Fectin or IT-PRO transfection reagents according to manufacturer’s instructions. 293-F cells were cultured in 1 mL volume in a 24-well shaken plate Density of cell culture was 1x106 cells/mL the day of transfection. Day 3 post-transfection, 20μL of supernatants were collected and SEAP activity was measured using SEAP assay Kit (#SP00500).
Hype-CHO
Hype-CHO Transfection Kit has been designed for large scale up transient transfection and high protein expression such as antibody. The system is optimized for maximum efficiency in all CHO-S cells. It has been used and validated with cells from different origins (CHO, CHO-S, rCHO or any CHO-related cells) cultured in suspension (flask, spinner and bioreactor) used to produce proteins.
Main caracteristics:
- Reach optimal Protein & Antibody production yield.
- Suitable for all CHO cells growing in suspension.
- Compatible with any synthetic or regular media used for protein production.
- Ideal for bioreactor, spinner or flasks.
- Animal origin free.
HYPE-CHO™ Transfection Kit achieves high antibody production level and largely outperforms other commercially available transfection reagents in CHO-S cells growing in suspension.
Figure 2: IgG Rabbit production after transient transfection of CHO-S cells with HYPE-CHO kit in 30 mL cell culture volume. Transfections were performed using 1.5 μg of plasmid DNA (antibody-expressing positive control vector, ThermoFisher) per mL of cell culture. 2 μL of HYPE-CHO reagent per μg of plasmid was used. CHO-S cells were cultured in 30 mL of medium in 125 mL shaken flasks. BCHO (1X final) was added to the cells 4h after the addition of the complexes (10μL per mL). Density of cell culture was 1x106 cells/mL the day of transfection. Day 3 and day 6 post-transfection, 500μL of supernatant was collected and IgG was measured with Easy-Titer Rabbit IgG Assay Kit (ThermoFisher).
Virus production
HYVIR
HYVIR™, is a novel lipid-based transfection kit dedicated to enhance viral particles production in HEK-293T. Its specific formulation allows an optimal plasmid compaction, protection and delivery rendering HYVIR ideal for the production of last generation of lentivirus.
Main caracteristics:
- Performant: reach high-titer functional lentiviral / virus production.
- Highly efficient: co-transfection of 4 plasmids at the same time.
- Reliable & Reproducible: easily scalable for production in large volumes and can be raised to superior grade.
- Ideal reagent for HEK293T cells transfection.
- Completely animal origin free.
HYVIR™ allows to reach high functional lentiviral production:
Figure 3: Results demonstrate the capacity of HYVIR™ to efficiently produce high yield of lentiviral particles compared to competitors.
Trusted by scientists and validated by thousands of publications in high-impact journals.
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