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Modeling memory B cell responses on an organ chip to assess mRNA vaccine boosts

BLOG > Publications & Citations > Modeling memory B cell responses on an organ chip to assess mRNA vaccine boosts

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Authors: Raphaël Jeger-Madiot et al.

Source: J Exp Med (2024) 221 (10): e20240289.

We’re delighted to share insights from a genuine study entitled "Modeling memory B cell responses in a lymphoid organ-chip to evaluate mRNA vaccine boosting" published in Journal of Experimental Medicine by Raphaël Jeger-Madiot et al:

"Predicting the immunogenicity of candidate vaccines in humans remains a challenge. To address this issue, we developed a lymphoid organ-chip (LO chip) model based on a microfluidic chip seeded with human PBMC at high density within a 3D collagen matrix. Perfusion of the SARS-CoV-2 spike protein mimicked a vaccine boost by inducing a massive amplification of spike-specific memory B cells, plasmablast differentiation, and spike-specific antibody secretion. Features of lymphoid tissue, including the formation of activated CD4+ T cell/B cell clusters and the emigration of matured plasmablasts, were recapitulated in the LO chip. Importantly, myeloid cells were competent at capturing and expressing mRNA vectored by lipid nanoparticles, enabling the assessment of responses to mRNA vaccines. Comparison of on-chip responses to Wuhan monovalent and Wuhan/Omicron bivalent mRNA vaccine boosts showed equivalent induction of Omicron neutralizing antibodies, pointing at immune imprinting as reported in vivo. The LO chip thus represents a versatile platform suited to the preclinical evaluation of vaccine-boosting strategies."

Congratulations to all the authors on this excellent article!

Our custom lnp platform provided GFP-LNP and OVA-LNP, used as control on lab-on-a-chip.

Read the paper See our custom LNP platform

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