ExploCap™ GFP saRNA
ExploCap SaGFP RNAs have been designed to produce high expression level of GFP protein with enhanced length of duration. OZB RNAs are produced by in vitro transcription and are co-transcriptionnaly capped using trinucleotides ExploCap analog and contain a poly(A) tail at the 3’ end. Sequences have been optimized to yield improved stability and performance.
OZB saGFP RNA has been constructed based on replicons of positive-sense ((+)-RNA) viruses (Venezuelan equine encephalitis Virus (VEEV)), where the coding sequence of the viral structural proteins is replaced with that of a gene of interest (GOI, here GFP), while retaining the coding sequences of non-structural proteins (NsPs), including the viral RNA-dependent RNA polymerase. Please note that OZB saRNAs encodes also for Puromycin Resistance Cassette, which does not alter the expression of the GOI but can be used for cell selection.
ExploCap GFP saRNAs:
- #MRNA119 does not bear any additional nucleotide modifications.
- #MRNA120 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).
Quality Controls
| Items | Specification | Standard QC | Superior Grade QC* |
|---|---|---|---|
| Integrity | Agarose gel mobility and fragment analyzer | ✓ | ✓ |
| Concentration | 1mg/ml +/- 5% | ✓ | ✓ |
| A260/280 |
>1.8 for Unmodified mRNAs >1.7 for chemically modified mRNAs |
✓ | ✓ |
| Sterility | Absence of bacterial growth at 37°C | ✓ | ✓ |
| Functionality** | Test for protein expression | ✓ | ✓ |
| Endotoxin | <0.5 EU/mL | ✓ | |
| dsRNA | <0.5% | ✓ |
*Our catalogue mRNAs undergo the standard QC. Superior Grade QC can be performed as an additional prestation. Contact us to get a quote.
** For reporter mRNAs and Spike SARS-Cov2 related mRNAs only for catalog mRNAs. Can be included in superior Grade QC.
Cap Modification: ExploCap™
Poly (A) Tail: Yes
Concentration: 1.0 mg/mL
Buffer: 1 mM Sodium Citrate, pH 6.4
Full length mRNA: 9821 nt
Size: 20 µg, 100µg and 1 mg
Storage: -80°C
Shipping: Dry ice (fee included in shipping cost)
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For bulk, please contact us: order@ozbiosciences.com
| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA119-20 |
saRNA GFP 20µg (unmodified)
|
$120.00
|
|
| MRNA119-100 |
saRNA GFP 100µg (unmodified)
|
$375.00
|
|
| MRNA119-1000 |
saRNA GFP 1mg (unmodified)
|
$1,975.00
|
|
| MRNA120-20 |
saRNA GFP 20µg (m5C)
|
$170.00
|
|
| MRNA120-100 |
saRNA GFP 100µg (m5C)
|
$400.00
|
|
| MRNA120-1000 |
saRNA GFP 1mg (m5C)
|
$2,075.00
|
|
Citation database
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