Cy3 GFP mRNA
GFP mRNAs have been designed to produce high expression level of GFP fluorescent protein.
OZB mRNAs are produced by in vitro transcription. mRNAs are stabilized at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end.
Sequences have been optimized to yield improved stability and performance.
GFP mRNA #MRNA15 does not bear any additional nucleotide modifications while #MRNA11 is modified with 5-methoxyuridine (5moU), #MRNA22 is modified with N1-methyl-pseudouridine to reduce innate immune response. #MRNA15B or 11B or 22B are labelled with Cy3 by replacing 15 % of UTP by UTP-Cy3.
Molecular weights:
- MRNA15B: 310699 g/mol.
- MRNA11B: 313708 g/mol.
- MRNA22B: 310681 g/mol.
Quality Control
Items | Specification | Standard QC | Superior Grade QC* |
---|---|---|---|
Appearance | Clear | ✓ | ✓ |
Integrity | One peak, right size | ✓ | ✓ |
One peak, right size | ✓ | ✓ | |
Concentration | 1mg/ml +/- 5% | ✓ | ✓ |
A260/280 | >1.8 for Unmod, >1.7 for modified | ✓ | ✓ |
Sterility | Absence of growth after 14 days | ✓ | ✓ |
Endotoxin | <0.5 EU/mL | ✓ | |
dsRNA | <0.5% | ✓ | |
Fonctionality | protein expression after transfection | ✓ | ✓ |
Fluorophore concentration | >/= 10pmol/µl | ✓ | ✓ |
*Contact us to get a quote.
We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.
Size: 100µg
For bulk, please contact us: order@ozbiosciences.com
mRNA length: 918 nt.
Storage: -80°C
Shipping : Dry ice (fee included in shipping cost)
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Catalog number | Unit Size | Price excl tax | Quantity / Buy |
MRNA11B |
(5moU) - 100 µg
|
$897.00
|
|
MRNA15B |
unmodified - 100 µg
|
$857.00
|
|
MRNA 22B |
(N1-mψ) - 100 µg
|
$897.00
|
|
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