HeLaFect
HeLaFect is a transfection reagent specifically developed for HeLa cell lineage transfection with high efficiency. HeLaFect is a lipid-based reagent based on the Tee-Technology (“Triggered Endosomal Escape”). The cationic design of HeLaFect reagent allows high nucleic acid compaction for an efficient transport into HeLa cells. This reagent is composed of biodegradable lipids leading to high viability and is ready-to-use.
HeLaFect transfection reagent benefits:
- Highly efficient: more than 80% of transfected HeLa cells
- Ready-to-use: no need for additional buffer
- Low nucleic acid amount - minimized toxicity
- High level of nucleic acid compaction
- Easy and straightforward protocol
- Compatible with any culture medium: medium change not required
Sizes:
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500µL: 125-250 transfections with 1µg of DNA
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1mL: 250-500 transfections with 1µg of DNA
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5mL: 1250-2500 transfections with 1µg of DNA
Storage: -20°C
Shipping Conditions: Room Temperature
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| HF20500 |
500 µL
|
$183.00
|
|
| HF21000 |
1 mL
|
$351.00
|
|
| HF25000 |
5 mL
|
$1,567.00
|
|
HF20500
500 µL
$183.00
HF21000
1 mL
$351.00
HF25000
5 mL
$1,567.00
Citation database
| NAME | MOLECULE VECTOR | PRODUCT | REFERENCE | |
| 786-O | DNA | HeLaFect | Chesnel F., Plos Genet. 2020 Nov 2;16(11):e1009183. | |
| HEK-293 | DNA | HeLaFect | Chesnel F., Plos Genet. 2020 Nov 2;16(11):e1009183. | |
| HEK-293 | DNA | HeLaFect | Soufi M., Neurol Genet. 2018 Oct 3;4(5):e274. | |
| HeLa | DNA | HeLaFect | Koblan LW., Nat Biotechnol . 2021 Jun 28. doi: 10.1038/s41587-021-00938-z. | |
| HeLa | DNA | HeLaFect | Chesnel F., Plos Genet. 2020 Nov 2;16(11):e1009183. | |
| HeLa | DNA/sgRNA | HeLaFect | Rees HA., Sci Adv. 2019 May 8;5(5):eaax5717. | |
| HeLa | DNA | HeLaFect | Le Bihan Y., Eur J of Med Chem 2019, doi.org/10.1016/j.ejmech.2019.05.041 | |
| HeLa | DNA | HeLaFect | Keskitalo S., Thesis 2017 - University of Helsinki | |
| HeLa | DNA | HeLaFect | Yadav L., Cell Syst. 2017 Mar 15. pii: S2405-4712(17)30083-2. | |
| HeLa | DNA | HeLaFect | Heikkinen T., Hum Mutat. 2017 Jan 5. |
