HeLaFect

HeLaFect is a transfection reagent specifically developed for HeLa cell lineage transfection with high efficiency. HeLaFect is a lipid-based reagent based on the Tee-Technology (“Triggered Endosomal Escape”). The cationic design of HeLaFect reagent allows high nucleic acid compaction for an efficient transport into HeLa cells. This reagent is composed of biodegradable lipids leading to high viability and is ready-to-use.

HeLaFect transfection reagent benefits:

Highly efficient: more than 80% of transfected HeLa cells
Ready-to-use: no need for additional buffer
Low nucleic acid amount - minimized toxicity
High level of nucleic acid compaction
Easy and straightforward protocol
Compatible with any culture medium: medium change not required

Sizes:

500µL: 125-250 transfections with 1µg of DNA
1mL: 250-500 transfections with 1µg of DNA
5mL: 1250-2500 transfections with 1µg of DNA

Storage: -20°C

Shipping Conditions: Room Temperature

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Catalog number	Unit Size	Price excl tax	Quantity / Buy
HF20500	500 µL	$183.00
HF21000	1 mL	$351.00
HF25000	5 mL	$1,567.00

Catalog number
Unit Sizer
Price excl tax
Quantity / Buy

HF20500

500 µL

$183.00

HF21000

1 mL

$351.00

HF25000

5 mL

$1,567.00

Citation database

-28,00 MKN

-304,00 ECV

-45,00 MKN

-7,00 MCF

-7,00 MKN

-7402,00 BEL

-77,00 SHP

1,00 MNT

1321N1

13762 Mat B III

143B

15P-1

16HBE

184BJ

208,00 F

2PK3

32Dcl3

3A6

3T3

3T3-J2

3T3-L1

3T6

3Y1

44S

4T1

5637

59M

661W

7,00 MCF

786-O

804G

8505C

9,00 F

9HTE

A10

A1207

A172

A20

A204

A2058

A2780

A2780/AD

A375

A431

A498

A549

A673

A72

A7r5

A875

AB1

Abdominal cavity

AC16

ACHN

Adherent gastric cells

Adipocytes

AdSC

AE-1

AFSC

AKR

all cells

Amnion cells

Amphophoenix cells

Anjou 65

Anterior pituitary gonadotropes

Aortic root

AR4-2J

ARPE-19

Astrocyte derived cells

Astrocytes

Astrocytesﾠ: neural stem cell derived

ATDC5

AtT-20

AU-565

Axons

B Lymphocytes

B-TC3

B104

B11

B16-F10

B35

B65

B82

B95a

Ba/F3

BAC

BAEC

BC3H1

BCEC-1

BE(2)-C

BE(2)-M17

Beas-2B

BEC

BeWo

BFC012

BHK-21

BIU-87

BJAB

BLCL

BLM

Bm5

BMEC – Brain microvascular endothelial cells

BMSC

BNL CL.2

Bone marrow cells

Bone Marrow derived macrophages

Bone marrow derived macrophages – BMDM

Bone marrow derived stromal cells

bone marrow monocytes

Bone marrow mononuclear cells

Bowes melanoma cells

Brain

BRCA cell lines

Breast cancer cell lines

BRL-AG-3C

BSC-1

BSC-40

BSM

BSR-T7

BT-20

BT-474

BT-477

BT-549

BTK143

BV-2

C-33A

C127

C13-NJ

C1R

C20

C272

C2C12

C3H 10T1/2

C57MG

C6/36

CaCo-2

CAK

Cal120

Cal27

Cal51

Calu-1

Calu-3

Calu-6

CAMA-1

Capan-2

Cardiac progenitor

Cardic cells

Cardiomyocytes

Cardiomyocytes cell line

Carotid artery smooth muscle

CaSki

CATH.a

CCD-1064sk

CD34+

CEF

CEM

CEMx174

Cf2Th

CFK2

CG-4

CHLA-15

CHLA-20

CHO

CHO-IG1

CHO-IG3

CHO-K1

CHO: suspension

Chondrocytes

Chromaffin cells

CHU-2

CJ7

CL7.1

Cl8

CLU-301

CMT-93

COH

Colo205

Colon cancer cells

Colonic endothelial cells

COS-1

COS-7

COV362

CP70

cPTC

CRFK

CS-1

CT26

CT51

CT60

CTLL-2

CV-1

CWR22Rv1

D3H2LN

Daoy

DAUDI

DC2.4

Dendritic cells

DF1

Distal convulated tubules

DLD-1

DRG cells

DT40

DU145

Ductal adenocarcinoma

E14TG2a

EA.hy926

Ear artery

EBC-1

ECC-1

EL-4

Embryonic stem cells

Endometrial stromal cells

Endothelial cells

Endothelial cells : Aorta

Endothelial colony forming

Endothelial Progenitors

Epicardial Mesothelial cells

Epithelial cells

Epithelial Lung cells

Epithelial organoid

EPP85-181

ES Embryonic Stem

ES-2

ES-D3

Ex vivo: AGM

Ex vivo: Aorta

Ex vivo: Brain Slice

Ex vivo: Kidney cryosection

Ex vivo: Retinal Explant

Ex vivo: Tendon

Ex vivo: Urogenital ridges

Ex vivo : pancreatic section

Expi293

F442-A

FaDu

FGC-4

Fibroblast like synoviocytes

Fibroblasts

Fibrochondrocytes

Fibroid Stem Cells

Fibrosarcoma

FL5.12

FM3A

Foetal neurons

Foreskin Fibroblast

FSDC

FTO-2B

G401

G402

Gastric gland

Gastric myofibroblasts

Gastric Parietal Cells

GC-2spd (ts)

GD25

GDTC

GH3

GH4C1

GL261

Glioblastoma

Glioma cells

Glioma Stem cells

Granta-519

Granulosa cells

GT1

GTL-16

H-500

H1299

H13

H146

H1792

H187

H19-7

H226

H23

H292

H295R

H35

H441

H4IIE

H661

H9c2

HA847

HaCaT

HAPI

HB60

HBEC

HBL-100

HBMEC

HBTE

HC11

HCA-7

HCAEC

hCBMC

HCC-1806

HCC-1954

HCC-38

HCC-4006

HCEn

HCT116

HCT15

HD180

HDF

HEC-1-A

HEC-1-B

HEK-293

HEK-293: suspension

HEL

HeLa

Hematopoietic Stem Cells

Hep2

Hep3B

Hepa 1-6

Hepatic Stellate Cells

hepatocellular carcinoma

Hepatocytes

HepG2

HepG3

hESC

HFF

HFF1

HFFF2

HiB5

High 5

hiPSC

HIT-T15

HITB5

HK2

hKC

HL-1

HL-5

HL-60

HMC3

HMCB

HMEC

HMEC-1

HMy2.C1R

HN12

HNP1

HNSCC

HOS

HOSE

HPAEC

HPE

HRE

Hs 578T

HS-578

HS-729T

Hs68

HSC

HSC-3

HSC39

HSC43

HSC45

HSC57

HSC59

HSG

HSPC

HSY-EB

HT1080

HT22

HT29

HTLA230

HUC-HSC

HUC-MSC

Huh-7

Human Aortic Smooth Muscle Cells

Human dermal fibroblast

human Embryonic Stem Cells

Hut 78

Hut-102

HuTu 80

HUVEC

IB3-1

IC-21

IDP

IEC-6

IGR-39

IGROV-1

Immature HSC

IMR-32

IMR-90

in vivo

in vivo Jejunum

in vivo Stomach

in vivo: Abdominal cavity

in vivo: Aortic root

in vivo: Brain

in vivo: Calvarial defect

in vivo: Ear artery

in vivo: Endothelial cells

in vivo: Fibrosarcoma

in vivo: hepatocellular carcinoma

in vivo: intramuscular injection

in vivo: left adductor muscle

in vivo: Lung

in vivo: Peri anal region

in vivo: Pupae

in vivo: sciatic nerve

in vivo: Skin Flap Model

in vivo: subarachnoid space

in vivo: subcutaneous injection

in vivo: Subcutaneous tumor

in vivo: Trypanosoma cruzi

in vivo : ear

in vivo : eye topical & IVT

in vivo : hippocampus

in vivo : inguinal adipose tissue

in vivo : intradermal

in vivo : intraperitoneal

in vivo : intravenous injection

INS-1

Intestinal Spheroids

IPC-298

iPS

IVEC

J558/L

J774

J82

JB6

JEG-3

Jejunum

JIMT-1

Jurkat

K46

K562

KC18-2-40

KD83

Kelly

Keratinocytes

KG-1a

KGN

Kidney Ascending Limb cells

Kidney section

KMM-1

KMS-11

KMS-12

KMS-5

KMST-6

KNRK

KPC

KPON

KS-1

KT-3

L-Cells

L-M(TK-)

L02

L1210

L6E9

L929

LAN-5

LEC

left adductor muscle

Liver cells

LK35.2

LLC

LLC-PK1

LMH

LN308

LN428

LNCap

LNT-229

LoVo

LS 174T

LSK / LKS

LSV5

LUHMES

Lung

Lymphoid (Oka)

M-1

M21

M21-L4

MA-10

Macrophages

Magi-CCR5

MC38

MC3T3-E1

Mca

McA-RH7777

MCF-10A

MCF-10DCIS

MCF-12A

MDA-MB-231

MDA-MB-435

MDA-MB-436

MDA-MB-453

MDA-MB-468

MDA-MB-549

MDAH-2274

MDBK

MDCK

ME-180

Medaka Cells

MEF

MEL

Melanocytes

Melanoma cells

Meningioma cells

mES

MES-SA

MESC2.10

Mesencephalic Cells

Mesenchymal Stem Cells

MeWo

MEXF-2090

Mf4/4

MG-63

MGC-803

MIA PaCa-2

Microglial

mIMCD

mIMCD-3

MIN6

Mitochondrial xenocybrid

MLE15

MLEC32

mlEnd

MM6

MN9D

MNNG/HOS

MoB

MOLT-4

Monocytes

Mononuclear cells

mouse Embryonic Stem Cells

MRC-5

MS-5

MS1

MSC

MT2

MT4

MTD-1A

MTLn3

MTSV1-7

Mutu I

Mutu III

Mv 1 Lu

Myoblasts

Myocytes

Myofibroblasts

Myometrial

N13

N1E-115

N2a

N87

NALM-6

NB324K

NB4

NBT-II

NCB-20

NCB-21

NCB-22

NCB-23

NCB-24

NCB-25

NCB-26

NCB-27

NCB-28

NCB-30

NCB-31

NCB-34

NCB-35

NCB-37

NCB-39

NCB-40

NCB-41

NCB-42

NCI-H2228

NCI-H23

NCI-H292

NCI-H295

NCI-H295R

NCI-H358

NCI-H441

NCI-H460

Neural Crest Cells

Neural Stem Cells

Neuro-2a / N2a

Neuroblastoma

Neuroblasts

Neurons

Neurons: amygdal

Neurons: Basal ganglia

Neurons: cerebellar granule (CGN)

Neurons: cervical ganglion

Neurons: cortical

Neurons: cortical embryonic DRG

Neurons: cortical-IPS derived

Neurons: dopaminergic neurons

Neurons: dopaminergic neurons (iPSC derived)

Neurons: dorsal root ganglion

Neurons: embryonic cortical

Neurons: embryonic motor neuron

Neurons: ganglia

Neurons: Hippocampal

Neurons: hypothalamic

Neurons: IPS derived from i3N

Neurons: motor neurons

Neurons: Motor neurons derived from ES cells

Neurons: Motor neurons derived from iPSC

Neurons: neocortical

Neurons: Neural Stem cells derived

Neurons: nodose ganglions

Neurons: septal

Neurons: Spinal cord

Neurons: Spiral Ganglion Neurons ﾖ SGN

Neurons: striatal

Neurons: superior cervical ganglia - SCG

Neurons: vagal afferents

Neurons: ventral mesencephalons

NG108-15

NHBE - normal human bronchial/tracheal epithelial cells

NHDF - normal human dermal fibroblasts

NHF3

NHFF

NIH-3T3

NIH-3T6

NIH: OVCAR-3

NMuMG

NRK - normal rat kidney epithelial cells

NS20Y

NSC19

NSC34

NTERA-2 cl.D1 (NT2/D1)

Nucleus pulposus

NYGM

O23

Olfactory Mucosal cells

OLHNI-2

Oligodendrocyte Precursors

Oligodendrocytes: NSC derived

OLN-93

OPAEC

Orbital fibroblast

Organoids

OS3

OSE

Osteoblasts

OV-90

Ovarian cells

OVCAR5

P19

P388D1

P3XAg8.653

P815

P825

PA-1

PA317

PAEC

Paju

Pam212

PANC-1

Pancreatic Islets

Pancreatic stelatte cells

Papilla cells

Parietal Cells

PBL

PBMC

PC-12

PC-3

PC-9

PCI-13

PDAC

PER.C6

Pex5+/-

Phoenix -ampho / Phoenix-Eco cells

Pinealocytes

Plat-E

PLB-985

PNT2

Pollen

Pre-B

PrEC

Progenitor cells (pancreatic)

Proximal Tubule Epithelial Cells-PTEC

PT67

PU5-1.8

Pupae

QGY-7703

QT35

QT6

R1/E

rabbit pleural mesothelial

RAEC

Raji

Ramos (RA1)

Rat adipocyte

Rat epithelial cells

Rat tracheal epithelial cells

Rat1

Rat2

Rat6

RAW 264.7

RCHO / RCHO-1

RCME - rabbit coronay microvessel endothelial cells

RDB

Retinal ganglion cells

RIE-1

RIN 1046-38

RK13

RKO

RL95-2

ROS 17/2.8

RPE

RPE-1

RPE.40

RW-4

RWPE1

S194

S2-CP10

S2-CP8

S2-CP9

S2/H

S2R+

S49.1

Saos-2

Satellite Cells

SC1 REW22

SCC-15

SCC-25

SCC-7

SCLC – small cell lung cancer

Sertoli Cell

sf21

sf21ae

sf9

SH-SY5Y

SHEP

SiHa

SIM-A9

SK-BR-3

SK-Hep-1

SK-LMS-1

SK-MEL-1

SK-MEL-28

SK-Mel-37

SK-N-AS

SK-N-BE(2)

SK-N-MC

SK-N-SH

SK-OV-3

SK-UT-1

Skin Flap Model

SL2

SM10

SMA-560

SMMC-7721

Smooth muscle cells

SNB-19

SNU-441

SNU-449

SolC1(-)

SOLD6

SP2/0-Ag14

Spermatozoa

Splenocytes

SPOC1

STC-1

Stomach

Striatal cells

Subcutaneous tumor

SUIT-2

SUP-T1

SV-MRC5

SVEC

SVEC-4

SVR

SW13

SW480

SW620

SW684

SW982

Synoviocytes

T lymphocytes

T lymphocytes CD4+

T lymphocytes CD8+

T-47D

T-Rex-293

T-Rex-CHO

T-Rex-HeLa

T24

T265

T3CHO/AT1A

T47D

T84

T98G

TA3/Ha

TE671

Tera-2

TGW-nu-1

Thick ascending limb cells

THP-1

Thymic Lymphoma

TIG-1

TIG-3

TIG3

TK-1

TKD2

TM4

TOV-LT

Trabecular Meshwork

Tracheal Myocyte

Trypanosoma cruzi

TS/A

tsA201

TYK-nu

TZM-bl

U-118

U-118 MG

U-138 MG

U-2-OS

U-251

U-251 MG

U-373

U-87

U-87 MG

U-937

UC 729-6

UMR 106

UMRC6

UOK 257

UOK 262

URO sa

Vero

Vero 10A1

Vero E6

VOT-E36

Vsa13

Vsa16

VSMC

W9.5

WEHI-231

WI-38

WM-1158

Wm-233-4

WM-239-A

WM-266-4

WM-852

XPC15

XPC16

Y-1

Y79

YH-13

YK6-1

Z-R75-1

ZR-75-1

3D Transfection DNA

3D Transfection siRNA

AAV

Adeno-Associated Virus (2)

Adenovirus

alphavirus

Antibody

cmRNA

Cotransfection DNA/DNA

Cotransfection DNA/siRNA

Cotransfection miRNA/LNA

Cotransfection mRNA/mRNA

Cotransfection siRNA/siRNA

DNA

DNA minicircles

DNA/sgRNA

dsRNA

flavivirus

Lentivirus

LNA

miRNA

morpholino

mRNA

Nanobodies

ODN

Other

poly I:C

Protein

RepRNA

Retrovirus

Retrovirus/Lentivirus

RNA

RNAi

RNP

RNP complexes

SARS-CoV-2

SF Virus

sgRNA

shRNA

shRNA encoded Lentivirus

shRNA encoded plasmid

shRNA-encoding plasmid

siRNA

Virus

3D-Fect

3D-FectIN

Ab-DeliverIN

AdenoBlast

AdenoMag

AlumVax Hydroxide

AlumVax Phosphate

BCA-PAK Protein Assay Kit

Bradford Protein Assay

BrainFectIN

CaLiVax-DOTAP Adjuvant

CaPo Transfection Kit

Cas13d mRNA

Cas9 mRNA

CFAVax

CNE-CPO Vaccine Adjuvant

CombiMag

COSFect

CPRG kit

CRE mRNA

Cy5 F-Luc mRNA

Cy5 GFP mRNA

DMNPE-caged Luciferin

DNA markers

DreamFect

DreamFect Gold

DreamFect Stem

dry ice

EcoTransfect

EPO mRNA

F-Luc mRNA

FluoMag

FluoProdige Assay Kit

FlyFectIN

G418 Sulfate

GelFectIN

Geneblasters

Genome Editing mRNA

GFP mRNA

Glial-Mag

h IL-2 mRNA

HA-H1N1 mRNA

HA-H3N2 mRNA

HeLaFect

Helix-IN Transfection Reagent

HYPE-293

Hype-5

HYPE-CHO

HYVIR™ Transfection Reagent

IFAVax

IgG-FITC

In vivo DogtorMag

In vivo PolyMag

In vivo SilenceMag

In vivo ViroMag

LDH Cytotoxicity Assay Kit

LentiBlast

LentiBlast Premium Superior Grade

LentiBlast Premium Transduction Enhancer

LipoMag Kit

LipoVax NTA(Ni) Vaccine Adjuvant

Luciferase Assay kit

Luciferin

Lullaby Stem

Lullaby Transfection Reagent

Mag4C Conservation Buffer

Mag4C-AD

Mag4C-LV

Magnetic plates

Magnetic Separation Racks

Magnetic sets

Magnetofectamine

Magnetofectamine O2

mCherry mRNA

MTT Cell Proliferation Kit

N SARS-Cov-2 mRNA

NanOZ empty-LNP

NanOZ LNP-mRNA(GFP)

NanOZ LNP-mRNA(Luc)

NanOZ LNP-mRNA(OVA)

NeuroMag

Nitric Oxide Detection Kit

ONPG kit

Optimized Cas9 Nuclease

OVA mRNA

OZBlue Cell Viability Kit

Plasmids - pVectOZ

PolyMag

PolyMag CRISPR

PolyMag Neo

PolyVax-CPO Vaccine Adjuvant

Pro-DeliverIN

ProDeliverIN CRISPR

R-Luc mRNA

R-Phycoerythrin

Reporter Gene mRNA (5moU)

Reporter Gene mRNA unmodified

Reprogramming mRNAs

RmesFect

RmesFect CRISPR

RmesFect Stem

ROS Detection Assay Kit

SEAP Assay kit

SelfMag Kit

Senescence Kit

si3D-Fect

si3D-FectIN

SilenceMag

siRNA-GFP / siRNA-Luc

Spike DELTA mRNA

Spike Omicron mRNA

Spike SARS CoV-2 mRNA

SqualVax

test Quai13

Tomato mRNA

VeroFect

Viro-PEG Lentivirus Concentrator

ViroMag

ViroMag CRISPR

ViroMag R/L

ViroMag Stem Transduction Enhancer

ViroMICST

ViroMICST Stem Transduction Enhancer

WST-8 Cell Proliferation Kit

X-Gal Staining kit

X-Gal Substrate

XPMag Explant Transfection Reagent

β-Gal mRNA

NAME	MOLECULE VECTOR	PRODUCT	REFERENCE
786-O	DNA	HeLaFect	Chesnel F., Plos Genet. 2020 Nov 2;16(11):e1009183.
HEK-293	DNA	HeLaFect	Chesnel F., Plos Genet. 2020 Nov 2;16(11):e1009183.
HEK-293	DNA	HeLaFect	Soufi M., Neurol Genet. 2018 Oct 3;4(5):e274.
HeLa	DNA	HeLaFect	Koblan LW., Nat Biotechnol . 2021 Jun 28. doi: 10.1038/s41587-021-00938-z.
HeLa	DNA	HeLaFect	Chesnel F., Plos Genet. 2020 Nov 2;16(11):e1009183.
HeLa	DNA/sgRNA	HeLaFect	Rees HA., Sci Adv. 2019 May 8;5(5):eaax5717.
HeLa	DNA	HeLaFect	Le Bihan Y., Eur J of Med Chem 2019, doi.org/10.1016/j.ejmech.2019.05.041
HeLa	DNA	HeLaFect	Keskitalo S., Thesis 2017 - University of Helsinki
HeLa	DNA	HeLaFect	Yadav L., Cell Syst. 2017 Mar 15. pii: S2405-4712(17)30083-2.
HeLa	DNA	HeLaFect	Heikkinen T., Hum Mutat. 2017 Jan 5.