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HeLaFect
  • HeLaFect
  • HeLaFect
  • HeLaFect

HeLaFect

HeLaFect is a transfection reagent specifically developed for HeLa cell lineage transfection with high efficiency. HeLaFect is a lipid-based reagent based on the Tee-Technology (“Triggered Endosomal Escape”). The cationic design of HeLaFect reagent allows high nucleic acid compaction for an efficient transport into HeLa cells. This reagent is composed of biodegradable lipids leading to high viability and is ready-to-use.

HeLaFect transfection reagent benefits:

  • Highly efficient: more than 80% of transfected HeLa cells
  • Ready-to-use: no need for additional buffer
  • Low nucleic acid amount - minimized toxicity
  • High level of nucleic acid compaction
  • Easy and straightforward protocol
  • Compatible with any culture medium: medium change not required
 

Sizes:

  • 500µL: 125-250 transfections with 1µg of DNA

  • 1mL: 250-500 transfections with 1µg of DNA

  • 5mL: 1250-2500 transfections with 1µg of DNA

 

Storage: -20°C

Shipping Conditions: Room Temperature

 

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Request a sample
Catalog number Unit Size Price excl tax Quantity / Buy
HF20500
500 µL
$195.00
HF21000
1 mL
$369.00
HF20500
500 µL
$195.00
HF21000
1 mL
$369.00

Citation database

1321N1
13762 Mat B III
143B
15P-1
16HBE
184BJ
208F
2PK3
32Dcl3
3A6
3T3
3T3-J2
3T3-L1
3T6
3Y1
44S
4T1
5637
59M
661W
786-O
804G
8505C
9HTE
A10
A1207
A172
A20
A204
A2058
A2780
A2780/AD
A375
A431
A498
A549
A6
A673
A72
A7r5
A875
AB1
Abdominal cavity
AC16
ACHN
Acinar Cells
Adherent gastric cells
Adipocytes
AdSC
AE-1
AFSC
AKR
All cells
AML
Amnion cells
Amphophoenix cells
Anjou 65
Anterior pituitary gonadotropes
Aortic root
AR4-2J
ARPE-19
Astrocyte derived cells
Astrocytes
Astrocytesﾠ: neural stem cell derived
ATDC5
AtT-20
AU-565
Axons
B Lymphocytes
B-TC3
B104
B11
B16-F1
B16-F10
B35
B6
B65
B82
B95a
Ba/F3
BAC
BAEC
BC3H1
BCEC-1
BE(2)-C
BE(2)-M17
Beas-2B
BEC
BEL-7402
BeWo
BFC012
BHK-21
BIU-87
BJ5ta
BJAB
BLCL
BLM
Bm5
BME6
BMEC – Brain microvascular endothelial cells
BMSC
BNL CL.2
Bone marrow cells
Bone Marrow derived macrophages
Bone marrow derived macrophages – BMDM
Bone marrow derived stromal cells
bone marrow monocytes
Bone marrow mononuclear cells
Bone Marrow Progenitor
Bowes melanoma cells
Brain
BRCA cell lines
Breast cancer cell lines
BRL-AG-3C
Bronchial epithelial cells
BSC-1
BSC-40
BSM
BSR-T7
BT-20
BT-474
BT-477
BT-549
BTK143
BV-2
C-33A
C127
C13-NJ
C1R
C20
C272
C2C12
C3H 10T1/2
C57MG
C6
C6/36
CaCo-2
CAK
CAL-1
Cal120
Cal27
Cal51
Calu-1
Calu-3
Calu-6
CAMA-1
CAP Spheroid
Capan-2
Cardiac progenitor
Cardic cells
Cardiomyocytes
Cardiomyocytes cell line
Carotid artery smooth muscle
CaSki
CATH.a
CCD-1064sk
CD34+
CEF
CEM
CEMx174
Cf2Th
CFK2
CG-4
CHLA-15
CHLA-20
CHO
CHO-IG1
CHO-IG3
CHO-K1
CHO: suspension
Chondrocytes
Chromaffin cells
CHU-2
CJ7
CL7.1
Cl8
CLU-301
CMT-93
COH
Colo205
Colon cancer cells
Colonic endothelial cells
COS-1
COS-7
COV362
CP70
cPTC
CRFK
CS-1
CT26
CT51
CT60
CTLL-2
CV-1
CWR22Rv1
D3
D3H2LN
D660
Daoy
DAUDI
DC2.4
Dendritic cells
DF1
Distal convulated tubules
DLD-1
DRG cells
DT40
DU145
Ductal adenocarcinoma
E14TG2a
EA.hy926
Ear artery
EBC-1
ECC-1
ECV-304
EL-4
Embryonic stem cells
EndoC beta H1
Endometrial stromal cells
Endothelial cells
Endothelial cells : Aorta
Endothelial colony forming
Endothelial Progenitors
Epicardial Mesothelial cells
Epithelial cells
Epithelial Lung cells
Epithelial organoid
EPP85-181
ER-Hoxb8
ES Embryonic Stem
ES-2
ES-D3
Ex vivo: AGM
Ex vivo: Aorta
Ex vivo: Brain Slice
Ex vivo: Kidney cryosection
Ex vivo: Retinal Explant
Ex vivo: retro-orbital injection
Ex vivo: Tendon
Ex vivo: Urogenital ridges
Ex vivo : pancreatic section
Ex vivo : porcine retinal explant
Ex vivo : porcine whole eye
Expi293
F442-A
F9
FaDu
FGC-4
Fibroblast cell line
Fibroblast like synoviocytes
Fibroblasts
Fibrochondrocytes
Fibroid Stem Cells
Fibrosarcoma
FL5.12
FM3A
Foetal neurons
Foreskin Fibroblast
FR
FSDC
FTO-2B
G401
G402
Gastric gland
Gastric myofibroblasts
Gastric Parietal Cells
GC-2spd (ts)
GD25
GDTC
GH3
GH4C1
GL261
Glioblastoma
Glioma cells
Glioma Stem cells
Granta-519
Granulosa cells
GT1
GTL-16
H-500
H1
H1299
H13
H146
H1792
H187
H19-7
H1994
H226
H23
H292
H295R
H35
H4
H441
H4IIE
H661
H7
H9
H9c2
HA847
HaCaT
HAEC
HAPI
HB60
HBEC
HBL-100
HBMEC
HBTE
HC11
HCA-7
HCAEC
hCBMC
HCC-1806
HCC-1954
HCC-38
HCC-4006
HCEn
HCT116
HCT15
HCT8
HD180
HDF
HEC-1-A
HEC-1-B
HEK-293
HEK-293: suspension
HEK-293A
HEK-293F
HEK-293T
HEL
HeLa
Hematopoietic Stem Cells
Hep2
Hep3B
Hepa 1-6
Hepatic Stellate Cells
hepatocellular carcinoma
Hepatocytes
HepG2
HepG3
hESC
HFF
HFF1
HFFF2
HiB5
High 5
hiPSC
HIT-T15
HITB5
HK2
hKC
HL-1
HL-5
HL-60
HMC3
HMCB
HMEC
HMEC-1
HMy2.C1R
HN12
HNP1
HNSCC
HOS
HOSE
HPAEC
HPE
HRE
Hs 578T
HS-578
HS-729T
Hs68
HSC
HSC-3
HSC39
HSC43
HSC45
HSC57
HSC59
HSG
HSPC
HSY-EB
HT1080
HT22
HT29
HTLA230
HUC-HSC
HUC-MSC
Huh-7
Human Aortic Smooth Muscle Cells
Human dermal fibroblast
human Embryonic Stem Cells
Hut 78
Hut-102
HuTu 80
HUVEC
IB3-1
IC-21
IDP
IE-6
IEC-6
IGR-39
IGROV-1
Immature HSC
IMR-32
IMR-90
in vivo
in vivo Jejunum
in vivo Stomach
in vivo: Abdominal cavity
in vivo: Aortic root
in vivo: Brain
in vivo: Calvarial defect
in vivo: Ear artery
in vivo: Endothelial cells
in vivo: Fibrosarcoma
in vivo: hepatocellular carcinoma
in vivo: intramuscular injection
in vivo: intratechal injection
in vivo: left adductor muscle
in vivo: Lung
in vivo: Peri anal region
in vivo: Pupae
in vivo: sciatic nerve
in vivo: Skin Flap Model
in vivo: subarachnoid space
in vivo: subcutaneous injection
in vivo: Subcutaneous tumor
in vivo: Trypanosoma cruzi
in vivo : brain
in vivo : ear
in vivo : eye topical & IVT
in vivo : hippocampus
in vivo : inguinal adipose tissue
in vivo : intra cerebellar
in vivo : intradermal
in vivo : intraperitoneal
in vivo : intratumoral
in vivo : intravenous injection
in vivo : superficial temporal vein
INS-1
Intestinal Spheroids
IPC-298
iPS
IVEC
J558/L
J774
J82
JB6
JEG-3
Jejunum
JIMT-1
Jurkat
JY
K46
K562
KB
Kc
KC18-2-40
KD83
Kelly
Keratinocytes
KG-1a
KGN
Kidney Ascending Limb cells
Kidney section
KM12
KMM-1
KMS-11
KMS-12
KMS-5
KMST-6
KNRK
KPC
KPON
KS
KS-1
KT-3
L-Cells
L-M(TK-)
L02
L1210
L2
L6
L6E9
L8
L929
LAN-5
LEC
left adductor muscle
Liver cells
LK35.2
LLC
LLC-PK1
LLE/LULS45
LMH
LN308
LN428
LNCap
LNT-229
LoVo
LS 174T
LS180
LSK / LKS
LSV5
LUHMES
LULS45
Lung
Lymphocytes
Lymphoid (Oka)
M-1
M1
M21
M21-L4
MA-10
Macrophages
Magi-CCR5
MC38
MC3T3-E1
Mca
McA-RH7777
MCF-10A
MCF-10DCIS
MCF-12A
MCF-7
MCF7
MDA-MB-231
MDA-MB-435
MDA-MB-436
MDA-MB-453
MDA-MB-468
MDA-MB-549
MDAH-2274
MDBK
MDCK
MDST8
ME-180
Medaka Cells
MEF
MEL
Melanocytes
Melanoma cells
Meningioma cells
mES
MES-SA
MESC2.10
Mesencephalic Cells
Mesenchymal Stem Cells
MeWo
MEXF-2090
Mf4/4
MG-63
MGC-803
MIA PaCa-2
Microglial
mIMCD
mIMCD-3
MIN6
Mitochondrial xenocybrid
MKN-28
MKN-45
MKN-7
MLE15
MLEC32
mlEnd
MM1S
MM6
MN9D
MNNG/HOS
MNT1
MoB
MOLT-4
Monocytes
Mononuclear cells
mouse Embryonic Stem Cells
MRC-5
MS-5
MS1
MSC
MT2
MT4
MTD-1A
MTLn3
MTSV1-7
Mutu I
Mutu III
Mv 1 Lu
Myeloid progenitor
Myoblasts
Myocytes
Myofibroblasts
Myometrial
N13
N1E-115
N2a
N87
N9
NALM-6
NB324K
NB4
NBT-II
NCB-20
NCB-21
NCB-22
NCB-23
NCB-24
NCB-25
NCB-26
NCB-27
NCB-28
NCB-30
NCB-31
NCB-34
NCB-35
NCB-37
NCB-39
NCB-40
NCB-41
NCB-42
NCI-H2228
NCI-H23
NCI-H292
NCI-H295
NCI-H295R
NCI-H358
NCI-H441
NCI-H460
Neural Crest Cells
Neural progenitor cells
Neural Stem Cells
Neuro-2a / N2a
Neuroblastoma
Neuroblasts
Neurons
Neurons: amygdal
Neurons: Basal ganglia
Neurons: cerebellar granule (CGN)
Neurons: cervical ganglion
Neurons: cortical
Neurons: cortical embryonic DRG
Neurons: cortical-IPS derived
Neurons: dopaminergic neurons
Neurons: dopaminergic neurons (iPSC derived)
Neurons: dorsal root ganglion
Neurons: embryonic cortical
Neurons: embryonic motor neuron
Neurons: excitatory
Neurons: excitatory – iPSC derived
Neurons: ganglia
Neurons: Hippocampal
Neurons: hypothalamic
Neurons: IPS derived from i3N
Neurons: motor neurons
Neurons: Motor neurons derived from ES cells
Neurons: Motor neurons derived from iPSC
Neurons: neocortical
Neurons: Neural Stem cells derived
Neurons: nodose ganglions
Neurons: septal
Neurons: Spinal cord
Neurons: Spiral Ganglion Neurons ﾖ SGN
Neurons: striatal
Neurons: superior cervical ganglia - SCG
Neurons: sympathetic
Neurons: vagal afferents
Neurons: ventral mesencephalons
NG108-15
NHBE - normal human bronchial/tracheal epithelial cells
NHDF - normal human dermal fibroblasts
NHF3
NHFF
NIH-3T3
NIH-3T6
NIH: OVCAR-3
NLSm cells
NMuMG
NRK - normal rat kidney epithelial cells
NS20Y
NSC19
NSC34
NTERA-2 cl.D1 (NT2/D1)
Nucleus pulposus
NYGM
O23
OK
Olfactory Mucosal cells
OLHNI-2
Oligodendrocyte Precursors
Oligodendrocytes: NSC derived
OLN-93
OPAEC
Orbital fibroblast
Organoids
OS3
OSE
Osteoblasts
OV-90
Ovarian cancer cell
Ovarian cells
OVCAR5
P19
P388D1
P3XAg8.653
P815
P825
PA-1
PA317
PAEC
Paju
Pam212
PANC-1
Pancreatic Islets
Pancreatic stelatte cells
Papilla cells
Parietal Cells
PASMC
PBL
PBMC
PC-12
PC-3
PC-9
PCI-13
PDAC
PER.C6
Pex5+/-
Phoenix -ampho / Phoenix-Eco cells
Pinealocytes
Plat-E
PLB-985
PNT2
Pollen
Pre-B
PrEC
Progenitor cells (pancreatic)
Proximal Tubule Epithelial Cells-PTEC
PT67
PU5-1.8
Pupae
QGY-7703
QT35
QT6
R1/E
rabbit pleural mesothelial
RAEC
Raji
Ramos (RA1)
Rat adipocyte
Rat epithelial cells
Rat tracheal epithelial cells
Rat1
Rat2
Rat6
RAW 264.7
RCHO / RCHO-1
RCME - rabbit coronay microvessel endothelial cells
RDB
Retinal ganglion cells
RIE-1
RIN 1046-38
RK13
RKO
RL95-2
ROS 17/2.8
RPE
RPE-1
RPE.40
RW-4
RWPE1
S194
S2
S2-CP10
S2-CP8
S2-CP9
S2/H
S2R+
S49.1
Saos-2
Satellite Cells
SC1 REW22
SCC-15
SCC-25
SCC-7
SCLC – small cell lung cancer
Sertoli Cell
sf21
sf21ae
sf9
SH-SY5Y
SHEP
SHP-77
SiHa
SIM-A9
SK-BR-3
SK-Hep-1
SK-LMS-1
SK-MEL-1
SK-MEL-28
SK-Mel-37
SK-N-AS
SK-N-BE(2)
SK-N-MC
SK-N-SH
SK-OV-3
SK-UT-1
Skin Flap Model
SL2
SM10
SMA-560
SMMC-7721
Smooth muscle cells
SNB-19
SNU-441
SNU-449
SolC1(-)
SOLD6
SP2/0-Ag14
Spermatozoa
Splenocytes
SPOC1
STC-1
Stomach
Striatal cells
Subcutaneous tumor
SUIT-2
SUP-T1
SV-MRC5
SVEC
SVEC-4
SVR
SW13
SW480
SW620
SW626
SW684
SW837
SW982
Synoviocytes
T lymphocytes
T lymphocytes CD4+
T lymphocytes CD8+
T-47D
T-Rex-293
T-Rex-CHO
T-Rex-HeLa
T24
T265
T3CHO/AT1A
T47D
T84
T98G
TA3/Ha
TDC Tendon derived cells
TE671
Tera-2
TGW-nu-1
Thick ascending limb cells
THP-1
Thymic Lymphoma
TIF
TIG-1
TIG-3
TIG3
TK-1
TKD2
TM4
TOV-112D
TOV-LT
Trabecular Meshwork
Tracheal Myocyte
Trypanosoma cruzi
TS/A
tsA201
TTF Tail Tendon Fascicels
TYK-nu
TZM-bl
U-118
U-118 MG
U-138 MG
U-2-OS
U-251
U-251 MG
U-373
U-87
U-87 MG
U-937
U266B1
UC 729-6
UMR 106
UMRC6
UOK 257
UOK 262
URO sa
Vero
Vero 10A1
Vero E6
VOT-E36
Vsa13
Vsa16
VSMC
W9.5
WEHI-231
WI-38
WM-1158
Wm-233-4
WM-239-A
WM-266-4
WM-852
XPC15
XPC16
Y-1
Y79
YH-13
YK6-1
Z-R75-1
ZR-75-1
0
3D Transfection DNA
3D Transfection siRNA
AAV
Adeno-Associated Virus (2)
Adenovirus
alphavirus
Antibody
brain extract
cmRNA
Cotransfection DNA/DNA
Cotransfection DNA/siRNA
Cotransfection miRNA/LNA
Cotransfection mRNA/mRNA
Cotransfection siRNA/siRNA
DENV2
DNA
DNA minicircles
DNA/sgRNA
dsRNA
flavivirus
Lentivirus
LNA
miRNA
morpholino
mRNA
Nanobodies
ODN
Other
poly I:C
Protein
RepRNA
Retrovirus
Retrovirus/Lentivirus
RNA
RNAi
RNP
RNP complexes
SARS-CoV-2
SF Virus
sgRNA
shRNA
shRNA encoded Lentivirus
shRNA encoded plasmid
shRNA-encoding plasmid
siRNA
tiRNA
Virus
3D-Fect
3D-FectIN
AAVBlast Transduction Enhancer
Ab-DeliverIN
AdenoBlast
AdenoMag
Alexa488 F-Luc mRNA
AlumVax Hydroxide
AlumVax Phosphate
BCA-PAK Protein Assay Kit
Bradford Protein Assay
BrainFectIN
c-Myc mRNA
CaLiVax-DOTAP Adjuvant
CaPo Transfection Kit
Capping Kit
Cas13d mRNA
Cas9 mRNA
CFAVax
CNE-CPO Vaccine Adjuvant
CombiMag
COSFect
CPRG kit
CRE mRNA
Cy3 F-Luc mRNA
Cy3 GFP mRNA
Cy5 F-Luc mRNA
Cy5 GFP mRNA
DMNPE-caged Luciferin
DNA markers
DreamFect
DreamFect Gold
DreamFect Stem
EcoTransfect
EPO mRNA
F-Luc mRNA
FluoMag
FluoProdige Assay Kit
FlyFectIN
G418 Sulfate
GelFectIN
Geneblasters
GFP mRNA
Glial-Mag
h IL-2 mRNA
h Sox2 mRNA
HA(-TMD)-H1N1 mRNA
HA(FL)-H1N1 mRNA
HA-H3N2 mRNA
HeLaFect
Helix-IN Transfection Reagent
HYPE-293
Hype-5
HYPE-CHO
HYVIR™ Transfection Reagent
IFAVax
IgG-FITC
In vivo DogtorMag
In vivo PolyMag
In vivo SilenceMag
In vivo ViroMag
IVT Kit
LDH Cytotoxicity Assay Kit
LentiBlast
LentiBlast Premium Superior Grade
LentiBlast Premium Transduction Enhancer
LipoMag Kit
LipoVax NTA(Ni)
LNP Selection Kit
Luciferase Assay kit
Luciferin
Lullaby Stem
Lullaby Transfection Reagent
Mag4C Conservation Buffer
Mag4C-AD
Mag4C-LV
Magnetic plates
Magnetic Separation Racks
Magnetic sets
Magnetofectamine
Magnetofectamine O2
mCherry mRNA
MTT Cell Proliferation Kit
N SARS-Cov-2 mRNA
NanOZ Dual Fluo LNP (DiO/Cy5-mRNAFLuc)
NanOZ empty-LNP
NanOZ Fluo LNP-DiI
NanOZ Fluo LNP-DiO
NanOZ Fluo LNP-DiR
NanOZ LNP-DIY(105S)
NanOZ LNP-DIY(FP105)
NanOZ LNP-DIY(SM102)
NanOZ LNP-DIY(SS1)
NanOZ LNP-mRNA (OVA)
NanOZ LNP-mRNA(GFP)
NanOZ LNP-mRNA(Luc)
NeuroMag
Nitric Oxide Detection Kit
ONPG kit
Optimized Cas9 Nuclease
OVA mRNA
OZBlue Cell Viability Kit
Plasmids - pVectOZ
Poly(A) Kit
PolyMag
PolyMag CRISPR
PolyMag Neo
PolyVax-CPO Vaccine Adjuvant
Pro-DeliverIN
Pro-DeliverIN CRISPR
R-Luc mRNA
R-Phycoerythrin
RmesFect
RmesFect Stem
RmesFect™ CRISPR
ROS Detection Assay Kit
saRNA GFP
saRNA Luc
SEAP Assay kit
SelfMag Kit
Senescence Kit
si3D-Fect
si3D-FectIN
SilenceMag
Spike DELTA mRNA
Spike Omicron mRNA
Spike SARS CoV-2 mRNA
SqualVax
SqualVax Vegetal
Tomato mRNA
VeroFect
Viro-PEG Lentivirus Concentrator
ViroMag
ViroMag CRISPR
ViroMag R/L
ViroMag Stem Transduction Enhancer
ViroMICST
ViroMICST Stem Transduction Enhancer
WST-8 Cell Proliferation Kit
X-Gal Staining kit
X-Gal Substrate
XPMag
β-Gal mRNA
NAME MOLECULE VECTOR PRODUCT REFERENCE
786-O DNA HeLaFect Chesnel F. Plos Genet. 2020 Nov 2;16(11):e1009183.
HEK-293 DNA HeLaFect Chesnel F. Plos Genet. 2020 Nov 2;16(11):e1009183.
HEK-293 DNA HeLaFect Soufi M. Neurol Genet. 2018 Oct 3;4(5):e274.
HeLa DNA HeLaFect Bernauer H. Biol Methods Protoc. 2024 Jan 19;9(1):bpae001.
HeLa DNA HeLaFect Bernauer H. Biol Methods Protoc. 2023 Dec 12;8(1):bpad030.
HeLa DNA HeLaFect Koblan LW. Nat Biotechnol . 2021 Jun 28. doi: 10.1038/s41587-021-00938-z.
HeLa DNA HeLaFect Chesnel F. Plos Genet. 2020 Nov 2;16(11):e1009183.
HeLa DNA/sgRNA HeLaFect Rees HA. Sci Adv. 2019 May 8;5(5):eaax5717.
HeLa DNA HeLaFect Le Bihan Y. Eur J of Med Chem 2019 doi.org/10.1016/j.ejmech.2019.05.041
HeLa DNA HeLaFect Keskitalo S. Thesis 2017 - University of Helsinki
HeLa DNA HeLaFect Yadav L. Cell Syst. 2017 Mar 15. pii: S2405-4712(17)30083-2.
HeLa DNA HeLaFect Heikkinen T. Hum Mutat. 2017 Jan 5.

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HeLaFect Results

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HeLaFect MSDS

Download 327.98KB

HelaFect Protocol

Download 879.94KB

HeLaFect rapid protocol

Download 749.77KB

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