Cy5 GFP mRNA

Cy5 GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It can be used as control of transfection efficiency. 

When labeled with Cy5, GFP mRNA can be easily traced to analyze mRNA delivery and translation efficiency.

OZ Biosciences mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNAs contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance.

Cy5 GFP mRNAs:

  • #MRNA15C does not bear any additional nucleotide modifications and is labelled with Cy5 (Sulfo-Cyanine5) by replacing 15 % of U.

  • #MRNA11C is modified with 5-methoxyuridine (5moU) and labelled with Cy5 (Sulfo-Cyanine5) by replacing 15 % of moU by UTP-Cy5.

  • #MRNA22C: is modified with N1-methylpseudouridine (N1-mψ) labelled with Cy5  (Sulfo-Cyanine5) by replacing 15 % of N1-mψ by UTP-Cy5.

 

Quality Control

Items

specification

Standard QC

Superior Grade QC*

Appearance

clear

Integrity

one peak, right size

one peak, right size

Concentration

1mg/ml +/- 5% 

A260/280

>1,8 for Unmod , >1,7 for modified

Sterility

absence of growth after 14 days

Endotoxin

<0,5 EU/ml

 

dsRNA

<0,05%

 

Fonctionality

protein expression after transfection

Fluorophore concentration

>/= 10pmol/µl

* Contact us to get a quote.

We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.

Size: 100µg

For bulk, please contact us: order@ozbiosciences.com

Storage: -80°C

Shipping : Dry ice (fee included in shipping cost)

 

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