Cy5 F-Luc mRNA
Cy5 F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
When labeled with Cy5, F-Luc mRNA can be easily traced to analyze mRNA delivery and translation efficiency.
OZ Biosciences mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNAs contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance.
Cy5 F-Luc mRNAs:
- #MRNA16c does not bear any additional nucleotide modifications and is labelled with Cy5 (Sulfo-Cyanine5) by replacing 15 % of U.
- #MRNA12c is modified with 5-methoxyuridine (5moU) and labelled with Cy5 (Sulfo-Cyanine5) by replacing 15 % of moU by UTP-Cy5.
- MRNA24C: is modified with N1-methylpseudouridine (N1-mψ) and labelled with Cy5 (Sulfo-Cyanine5) by replacing 15 % of N1-mψ by UTP-Cy5.
Quality Control
|
Items
|
specification
|
Standard QC |
Superior Grade QC* |
|
Appearance |
clear |
✓ |
✓ |
|
Integrity |
one peak, right size |
✓ |
✓ |
|
one peak, right size |
✓ |
✓ |
|
|
Concentration |
1mg/ml +/- 5% |
✓ |
✓ |
|
A260/280 |
>1,8 for Unmod , >1,7 for modified |
✓ |
✓ |
|
Sterility |
absence of growth after 14 days |
✓ |
✓ |
|
Endotoxin |
<0,5 EU/ml |
|
✓ |
|
dsRNA |
<0,05% |
|
✓ |
|
Fonctionality |
protein expression after transfection |
✓ |
✓ |
|
Fluorophore concentration |
>/= 10pmol/µl |
✓ |
✓ |
* Contact us to get a quote.
We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.
Size: 100µg
For bulk, please contact us: order@ozbiosciences.com
Storage: -80°C
Shipping : Dry ice (fee included in shipping cost)
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA16c-100 |
F-Luc mRNA (U-Cy5) 100 µg
|
$793.00
|
|
| MRNA12c-100 |
F-Luc mRNA (5moU/U-Cy5) 100 µg
|
$857.00
|
|
| MRNA24C-100 |
(N1-mψ) - 100 µg
|
$857.00
|
|
MRNA16c-100
F-Luc mRNA (U-Cy5) 100 µg
$793.00
MRNA12c-100
F-Luc mRNA (5moU/U-Cy5) 100 µg
$857.00
MRNA24C-100
(N1-mψ) - 100 µg
$857.00
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