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Alexa488 F-Luc mRNAs have been designed to produce high expression level of Firefly Luciferase protein.
When labeled with AlexaFluor488 (AF488) green fluorescent dye, F-Luc mRNA can be easily traced to analyze mRNA delivery and translation efficiency.
mRNAs are produced by in vitro transcription. mRNAs are stabilized at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end. Sequences have been optimized to yield improved stability and performance.
Alexa488 F-Luc mRNAs Catalog Number:
Quality Control
| Items | Specification | Standard QC | Superior Grade QC* |
|---|---|---|---|
| Integrity | Agarose gel mobility and HPLC | ✓ | ✓ |
| Concentration | 1mg/ml +/- 5% | ✓ | ✓ |
| A260/280 | >1.8 for Unmodified mRNAs, >1.7 for chemically modified mRNAs | ✓ | ✓ |
| Sterility | Absence of bacterial growth at 37°C | ✓ | ✓ |
| Functionality | Test for protein expression | ✓ | ✓ |
| Fluorophore concentration | >/= 10pmol/µl | ✓ | ✓ |
| Endotoxin | <0.5 EU/mL | ✓ | |
| dsRNA | <0.5% | ✓ |
*Our catalogue mRNAs undergo the standard QC. Superior Grade QC can be performed as an additional prestation. Contact us to get a quote.
We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.
Size: 100µg
For bulk, please contact us: order@ozbiosciences.com
Storage: -80°C
Shipping : Dry ice (fee included in shipping cost)
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA16A |
Unmodified Alexa488 F-Luc mRNA - 100 µg
|
$1,015.00
|
|
| MRNA12A |
5moU Alexa488 F-Luc mRNA - 100 µg
|
$1,070.00
|
|
| MRNA24A |
N1-mψ Alexa488 F-Luc mRNA - 100 µg
|
$1,070.00
|
|
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