Alexa488 F-Luc mRNA
Ready-to-use stabilized F-Luc mRNA
Concentration: 1.0 mg/mL in 1 mM Sodium Citrate, pH 6.4 mRNA length: 1872nt.
Molecular weights:
MRNA16: 603550 g/mol; MRNA16C: 630616 g/mol; MRNA16B: 660802 g/mol ; MRNA16A: 694200 g/mol
MRNA12: 611290 g/mol; MRNA12C: 636796 g/mol; MRNA12B: 644151 g/mol ; MRNA12A: 669033 g/mol
MRNA24: 607420 g/mol; MRNA24B: 637420 g/mol; MRNA24C: 640741 g/mol ; MRNA24A: 643953 g/mol
F-Luc mRNAs have been designed to produce high expression level of Firefly Luciferase protein.
OZB mRNAs are produced by in vitro transcription. mRNAs are stabilized at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end.
Sequences have been optimized to yield improved stability and performance. F-Luc mRNA #MRNA16 does not bear any additional nucleotide modifications while #MRNA12 is modified with 5-methoxyuridine (5moU), #MRNA24 is modified with N1-methyl-pseudouridine (N1-mψ) to reduce innate immune response. #MRNA16C or 12C or 24C are labelled with Cy5 by replacing 15 % of UTP by UTP-Cy5. #MRNA16B or 12B or 24B are labelled with Cy3 by replacing 15 % of UTP by UTP-Cy3 and #MRNA16A or 12A or 24A are labelled with AlexaFluor488 by replacing 15 % of UTP by UTP-AF488.
| Standard QC | Superior Grade QC* | |
| Fragment analyzer | ✔ | ✔ |
| Agarose gel | ✔ | ✔ |
| Concentration (260/280 & 260/230) | ✔ | ✔ |
| Sterility test | ✔ | ✔ |
| Transfection/Expression | ✔ | ✔ |
| Endotoxin | ✔ | |
| dsRNA | ✔ |
* Contact us to get a quote.
We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.
Size: 100µg
For bulk, please contact us: order@ozbiosciences.com
Storage: -80°C
mRNAs ARE SHIPPED WITH DRY ICE
Dry ice fee included in shipping cost
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA16A |
unmodified - 100 µg
|
$1,015.00
|
|
| MRNA12A |
(5moU) - 100 µg
|
$1,070.00
|
|
| MRNA 24A |
(N1-mψ) - 100 µg
|
$1,070.00
|
|
MRNA16A
unmodified - 100 µg
$1,015.00
MRNA12A
(5moU) - 100 µg
$1,070.00
MRNA 24A
(N1-mψ) - 100 µg
$1,070.00
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