LentiBlast Premium Superior Grade is the ideal reagent to enhance lentiviral infection and transduction in CD34+ hematopoietic stem cells, T lymphocytes as well as any other cells.
It is identical in synthesis and formulation to LentiBlast Premium Transduction Enhancer with advanced quality controls. It is designed for use in preclinical and early phase clinical trials and can be used as an ancillary material for cell & gene therapy.
Its patented chemical composition allows to simultaneously neutralize electrostatic repulsions between membrane and viral particles and to enhance viral fusion with cell membrane. Thanks to this favorable “membrane permeable effect”, LentiBlast Premium Superior Grade limits any transmembrane potential changes and thus facilitates virus entry.
Quality Control:
| Items | Specification | LentiBlast Premium | LentiBlast Premium SG |
|---|---|---|---|
| Sterility | Thioglycolate assay. Absence of fungal and bacterial contamination for 15 days | ✓ | ✓ |
| Biological Activity | Lentiviral transduction enhancement after 3 days on KG1a CD34+ cell line. Acceptance specification >80% | ✓ | ✓ |
| Chemical Characterization | Identification of signature peaks by ¹H and ¹³C NMR | ✓ | |
| Purity | Single peak observed in SEC chromatogram | ✓ | |
| Mycoplasma | Absence of Mycoplasma according to USP <63>* | ✓ | |
| Endotoxin | Presence of low levels of bacterial endotoxins as defined by USP <85>** | ✓ |
*USP <63> Mycoplasma Tests: a new regulation for mycoplasma testing. ** USP <85> Bacterial Endotoxins Test
Sizes:
1 500 µL: For up to 300 transductions in a 24-well plate.
5 000 µL: For up to 1 000 transductions in a 24-well plate.
Storage: -20°C
Shipping Conditions : Room temperature
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| LBPSG50 |
5 mL
|
$1,945.00
|
|
| LBPSG15 |
1500 µL
|
$751.00
|
|
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