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I-MICST Technology

PRINCIPLE

Integrated Magnetic Immuno-Cell transduction

i-MICST™ Technology (integrated Magnetic Immuno-Cell Sorting and Transfection/ Transduction) is a new platform that allows to genetically modify cells directly on magnetic cell purification columns. This technology combines cell isolation and genetic modification in one simple, efficient and reliable integrated system. Designed for i-MICST™ Technology, the Viro-MICST™ reagent allows the efficient and specific transduction of target cells directly on magnetic cell-purification columns.
Designed for stem cells, we developed Viro-MICST Stem Transduction Enhancer.

WHY USE Viro-MICST™?

Viro-MICST™ leads to an increase in the transduction efficiency with low-titer virus preparations compared to regular transduction methods and allows you to reduce cell manipulation steps and save time as well as vector material.

  • Isolation and transduction of cells in one reliable integrated system.

  • High and increased transduction efficiency with low MOI.

  • Acceleration of the transduction process and synchronization of adsorption.

  • Ideal for sensitive cell types such as primary and stem cells.

HOW DOES IT WORK?

i-MICST™ Technology requires:

  • Magnetic cell separation systems (not provided by OZ Biosciences).

  • The Viro-MICST™ reagent for capturing virus and infecting cells within the magnetic cell purification column.

Viro-MICST™ is a new specific magnetic nanoparticle formulation evolved from our Magnetofection™ Technology developed in association with MACS® technology* from Miltenyi Biotec.

Viro-MICST™ binds to viruses. As both magnetically labeled target cells and virus-Viro-MICST™ complexes are retained by the magnetic field into the column, the viruses can interact and infect target cells with high efficiency.

The i-MICST™ protocol is depicted as a two-steps process:

  1. Pre-enrichment step of magnetically labeled cells on non-modified column(s).

  2. Viro-MICST™ procedure. This step allows reaching high purity and simultaneously infecting the target cell population. (cf. fig.1)

* MACS® is a registered trademark owned by Miltenyi Biotec GmbH and the use of MACS® columns is a proprietary and patented technology. For any further licensed of MACS® system, please contact Miltenyi Biotec.