NanOZmix-DIY-105S
Ready-to-use dried lipid mix at the total lipid concentration of 25 mM when reconstituted in 1mL Ethanol, for LNP-siRNA, LNP-Oligonucleotide and LNP-miRNA formulation.
LNPs represent the most effective and safe delivery systems for the translational success of nucleic acid drugs. LNPs are lipidic spherical vesicles formed by a combination of four main components: an ionizable cationic lipid, a helper phospholipid, cholesterol & a pegylated lipid, each having distinct functions (Fig.1). LNPs not only protect RNA from degradation, but also facilitate intracellular uptake and thus potentiate its efficacy.
LNP/RNA systems self-assemble via electrostatic interactions between negatively charged RNA and ionizable cationic lipids.
NanOZmix-DIY-105S is designed for the development of LNP by customer at their ease by using different formulation methods at different N/P ratio. With our NanOZmix-DIY-105S system, customers can produce themselves a monodisperse LNP encapsulating mRNA within the size range of 50 nm to 200 nm through microfluidic/ impingement jets mixing (IJM)/T-junction mixing technology with an encapsulation efficiency of 80-96 % under optimized conditions.
Kit contents:
- LDIY11000: Dried 25 mM lipid mix having composition as described in table below:
| Lipid Mix Components | Molecular Weight | Molar Ratio | mM | mg |
| FP105s | 731.2 | 50 | 12.5 | 9.40 |
| DSPC | 790.17 | 10 | 2.5 | 1.95 |
| Cholesterol | 386.65 | 38.5 | 9.62 | 3.72 |
| DMG-PEG 2000 | 2509.2 | 1.5 | 0.38 | 0.94 |
| Total | 100 | 25 | 16.01 |
Storage:
NanOZmix-DIY-105S must be stored at -20°C as a dried film and at -80°C as ethanolic solution. Dried lipidic film can be stored for 6 months whereas ethanolic solution must be used within one month..
Preparation of 25 mM ethanolic lipid mix stock
Bring the dry film of NanOZmix-DIY-105 at room temperature from -20°C. Add 1 mL of ethanol in it and vortex it until you see clear solution. If you see sign of precipitation, try to sonicate or heat the ethanolic solution at 60°C for 1-2 min and vortex again.
Shipping Conditions: Dry Ice.
Dry ice fee included in shipping cost.
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| LDIY105S |
25mM
|
$600.00
|
|
LDIY105S
25mM
$600.00
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