Poly(A) Kit
Ready-to-use kit for poly(A) tailing of capped or non-capped mRNA.
This kit is designed for the enzymatic addition of a poly(A) tail to the 3’-hydroxyl termini of capped or non-capped RNAs independently of the template. Poly(A) Tailing Kit allows the addition of approximately 200 nucleotides per reaction (based on 100µg of RNA) in 45 minutes at 37°C.Presence of a poly(A) tail is known to enhance mRNA translation, stability and nucleo-cytoplasmic transport.
Kit contents:
- Poly(A) polymerase 5 u/µl, 75µl
- ATP (10 mM), 500µl
- RNase Inhibitor 40 u/µl, 50µl
- Polyadenylation Buffer 10X, 500µl
- Nuclease-free water, 2ml
Material not provided with the kit:
- mRNA to be polyadenylated
- 70% ethanol for RNA washing
- Nuclease-free buffers (1mM citrate buffer pH 6.4, TE buffer or RNAse-free water) for RNA resuspension.
- Materials or kits for purification of the RNA product
Storage: Upon receipt and for long-term use, store the kit at -20°C. Kit’s components are stable for at least 1 year at the recommended storage temperature.
Shipping condition: The Poly(A) tailing kit is shipped with dry ice.
| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| POLYA-K25 |
25 reactions
|
$320.00
|
|
POLYA-K25
25 reactions
$320.00
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