NanOZ Fluo LNP-DiO
Ready-to-use DiO-labeled LNPs for in vitro cellular and subcellular distribution and in vivo imaging..
Concentration: 1 mole % of DiO in 4.5 mM of LNPs in PBS with 10 % sucrose.
LNPs represent one of the most effective and safe delivery systems for the translational success of nucleic acid drugs. LNPs are lipidic spherical vesicles formed by a combination of four main components: an ionizable cationic lipid, a helper phospholipid, cholesterol & a pegylated lipid, each having distinct functions. LNPs not only protect RNA from degradation, but also facilitate intracellular uptake and thus potentiate its efficacy.
NanOZ Fluo LNP-DiO (Fig.1) is designed for the effective monitoring and imaging of intracellular trafficking and biodistribution of LNPs in vitro and in vivo respectively.
NanOZ Fluo LNP-DiO contains a lipophilic carbocyanine dye DiO having an excitation max. at 487 nm, and emission max. at 501 nm.
Kit content:
LFO11000: 1mL (4*250μL) of LNP-DiO, (4.5 mM) having composition as described in table below:
| Lipid mix components | Molecular weight | Molar ratio | mM |
| FP105 | 731.2 | 50 | 2.29 |
|
DSPC |
790.17 | 9 | 0.41 |
| Cholesterol | 386.65 | 38.5 | 1.76 |
| DMG-PEG 2000 | 2509.2 | 1.5 | 0.07 |
| DiR | 1013 | 1 | 0.05 |
| Total | 100 | 4.5 |
| Standard QC | Superior Grade QC* | |
| Size | ✔ | ✔ |
| Charge | ✔ | ✔ |
| Encapsulation efficiency | ✔ | ✔ |
| Sterility | ✔ | ✔ |
| Fluo detection | ✔ | ✔ |
| Endotoxin | ✔ | |
| Mycoplasma detection | ✔ | |
| Lipid content | ✔ |
* Contact us to get a quote.
Storage
NanOZ Fluo LNP-DiO must be stored at -80°C. We recommend to minimize freeze-thaw cycles to preserve LNPs integrity.
| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| LFO10500 |
0,5 ml
|
$615.00
|
|
| LFO11000 |
1ml
|
$1,025.00
|
|
| LFO15000 |
5 ml
|
$4,100.00
|
|
LFO10500
0,5 ml
$615.00
LFO11000
1ml
$1,025.00
LFO15000
5 ml
$4,100.00
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