GFP mRNA
GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It can be used as control of transfection efficiency.
OZ Biosciences mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNAs contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance.
GFP mRNAs:
- #MRNA15 does not bear any additional nucleotide modifications.
- #MRNA11 is modified with 5-methoxyuridine (5moU) to reduce innate immune response.
- #MRNA22 is modified with N1-methyl-pseudouridine (N1-mψ) to reduce innate immune response.
- #MRNA15c and #MRNA11c are labelled with Cy5 (Sulfo-Cyanine5) by replacing 15 % of U or moU by UTP-Cy5.
Quality Control
Items
|
specification
|
Standard QC |
Superior Grade QC* |
Appearance |
clear |
✓ |
✓ |
Integrity |
one peak, right size |
✓ |
✓ |
one peak, right size |
✓ |
✓ |
|
Concentration |
1mg/ml +/- 5% |
✓ |
✓ |
A260/280 |
>1,8 for Unmod , >1,7 for modified |
✓ |
✓ |
Sterility |
absence of growth after 14 days |
✓ |
✓ |
Endotoxin |
<0,5 EU/ml |
|
✓ |
dsRNA |
<0,05% |
|
✓ |
Fonctionality |
protein expression after transfection |
✓ |
✓ |
Fluorophore concentration |
>/= 10pmol/µl |
✓ |
✓ |
* Contact us to get a quote.
We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.
Size: 20 µg, 100µg and 1 mg
For bulk, please contact us: order@ozbiosciences.com
Storage: -80°C
Shipping: Dry ice (fee included in shipping cost)
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Catalog number | Unit Size | Price excl tax | Quantity / Buy |
MRNA15-20 |
mRNA GFP 20 µg
|
$185.00
|
|
MRNA15-100 |
mRNA GFP 100µg
|
$351.00
|
|
MRNA15-1000 |
mRNA GFP 1 mg
|
$1,887.00
|
|
MRNA11-20 |
mRNA GFP (5moU) 20 µg
|
$184.00
|
|
MRNA11-100 |
mRNA GFP (5moU) 100 µg
|
$415.00
|
|
MRNA11-1000 |
mRNA GFP (5moU) 1mg
|
$2,223.00
|
|
MRNA15c-100 |
GFP mRNA (U-Cy5) 100 µg
|
$841.00
|
|
MRNA11c-100 |
GFP mRNA (5moU/U-Cy5) 100 µg
|
$897.00
|
|
MRNA22-20 |
GFP mRNA (N1-mψ) 20 µg
|
$206.00
|
|
MRNA22-100 |
GFP mRNA (N1-mψ) 100 µg
|
$436.00
|
|
MRNA22-1000 |
GFP mRNA (N1-mψ) 1 mg
|
$2,244.00
|
|
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