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Cholesterol in mRNA-Lipid Nanoparticles can be Replaced with the Synthetic MMG-1

BLOG > Publications & Citations > Cholesterol in mRNA-Lipid Nanoparticles can be Replaced with the Synthetic MMG-1

Spike SARS CoV-2 mRNA

Authors: A. G. Lokras, S. S. Baghel, R. F. Jensen, et al.

Source: Adv. Funct. Mater. (2025): e05627.

We're delighted to share insights from a recent study entitled "Cholesterol in mRNA-Lipid Nanoparticles can be Replaced with the Synthetic Mycobacterial Monomycoloyl Glycerol Analogue MMG-1" published in Advanced Functional Materials by Abhijeet G. Lokras et al.

They demonstrated that replacing cholesterol in mRNA-Lipid Nanoparticles (LNPs) with the immunopotentiating lipid MMG-1 creates a novel, sterol-free delivery system. This innovation maintains physicochemical stability and in vivo protein expression while significantly improving transfection efficiency in immune cells like T cells, B cells, and macrophages. Notably, MMG-1-based LNPs induced robust SARS-CoV-2 spike protein-specific T-cell and virus-neutralizing antibody responses in mice, comparable to cholesterol-based formulations. Furthermore, in Syrian golden hamsters, these next-generation LNPs enhanced S-specific IgG titers and reduced SARS-CoV-2 viral load in the nasal cavity, offering crucial insights for improved mRNA vaccine design.

Congratulations to all the authors on this excellent article!

Our Spike SARS-CoV-2 mRNA fully substituted with N1-methylpseudouridine was used for the in vivo immunogenicity studies, enabling high levels of spike protein translation essential for target-specific immunity and protection against SARS-CoV-2.

Read the article See our Spike SARS-CoV-2 mRNA

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