BLOG > Publications & Citations > Multiple LDLR family members act as entry receptors for yellow fever virus
Authors: Chong, Z., Hui, S., Qiu, X. et al.
Source: Nature (2025).
We are delighted to share insights from a groundbreaking study entitled "Multiple LDLR family members act as entry receptors for yellow fever virus" published in Nature by Zhenlu Chong et al.
They conducted a pivotal study using a CRISPR–Cas9 screen to clarify the long-unanswered question of Yellow Fever Virus (YFV) entry receptors.
Their comprehensive research established that YFV utilizes multiple members of the Low-Density Lipoprotein Receptor (LDLR) family, specifically LRP4, LRP1, and VLDLR, to enable infection and pathogenesis. Mechanistically, LRP4 promotes YFV entry through its LDLR type A (LA) domain binding to domain III of the YFV envelope protein (E-DIII). This finding is key to understanding YFV tropism and developing countermeasures against emerging orthoflaviviruses.
To generate the necessary chimeric YFV/Binjari viruses for their detailed protein binding experiments, the circular CPER product was transfected into C6/36 Aedes albopictus cells.
Congratulations to all authors for this great article.
Our FlyFectIN was utilized by the authors for this crucial step in assembling the chimeric Orthoflavivirus particles. The ability to generate these complex viral platforms efficiently was instrumental in performing the detailed protein binding and neutralization assays that confirmed the role of these new receptors.
Read the article See FlyFectIN
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