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In-vivo delivery - fAQ & Tips

 In vivo Transfection Reagents

How to choose between In vivo PolyMag or In vivo DogtorMag?

In vivo PolyMag is a polymer-based magnetic nanoparticles, In vivo DogtorMag is a lipid-based magnetic nanoparticles. They are both made for nucleic acid transfection and the choice will depend on customer preference (polymer or lipid) as well as on existing data. There are many results with both; you can get them on our website. In some models in vivo PolyMag has been successful and in other in vivo DogtorMag.

Why the In vivo PolyMag Kit does not include in vivo CombiMag while In vivo DogtorMag includes in vivo CombiMag ?

In vivo PolyMag is an all-in-one formulation, meaning that it is ready to be mixed with nucleic acids and injected. In contrast, in vivo DogtorMag contains the lipid-based reagent that must be combined to the magnetic nanoparticles (in vivo CombiMag). 

What kind of magnets are used for in vivo Magnetofection, e.g. into the brain ?

The magnets used in vivo are: 1 extra small cylinder (ø 2 mm), 1 small cylinder (ø 5 mm), 1 cylinder (ø 10 mm) or 1 square (18x18 mm) magnet. You can also order only square magnets (packaged by 4) or only disc magnets (packaged by 4 of each). Each kit contains a complete magnet set (with 4 sizes of magnet), you can also order an extra magnet set. Depending on your application, the animal, the location of the targeting you can use any of the previous magnets. Nevertheless, for small animals we recommend either the extra small cylinder (2mm) or the small cylinder (5mm). The last one works very well to target embryo rat cortex for example.

Can in vivo PolyMag be used for siRNA and microRNA ?

Definitively yes, in vivo Polymag can be used for any nucleic acid; in general if a transfection reagent is able to transport long nucleic acids (DNA) it should be able to transport short nucleic acids.

Can we use in vivo Polymag for viral transduction, in vivo ?

We do not recommend using in vivo PolyMag for viral transduction in vivo. Magnetic nanoparticles in PolyMag have been designed to highly compact DNA and may be incompatible with viral applications. We have developed in vivo ViroMag that is dedicated to viral transduction in vivo. That’s why, for in vivo viral applications we will recommend in vivo ViroMag.