NanOZ Dual Fluo LNP (DiO/Cy5-mRNAFLuc)
Ready-to-use DiO-labeled and Cy5-mRNAFLuc encapsulated LNPs for in vitro cellular and subcellular distribution and in vivo imaging.
Concentration: 0.1 mg/mL Cy5-mRNAFLuc mRNA encapsulated in 4.5 mM of LNPs containing 1 mole % of DiO in PBS with 10 % sucrose.
LNPs represent one of the most effective and safe delivery systems for the translational success of nucleic acid drugs. LNPs are lipidic spherical vesicles formed by a combination of four main components: an ionizable cationic lipid, a helper phospholipid, cholesterol & a pegylated lipid, each having distinct functions. LNPs not only protect RNA from degradation, but also facilitate intracellular uptake and thus potentiate its efficacy.
NanOZ Dual Fluo LNP-DiO_Cy5-mRNAFLuc (Fig.1) is designed for the effective monitoring and imaging of intracellular trafficking and biodistribution of LNPs in vitro and in vivo respectively. NanOZ Dual Fluo LNP-DiO_ Cy5-mRNAFLuc contains a lipophilic carbocyanine dye DiO having an excitation max. at 487 nm, and emission max. at 501 nm and Cy5 mRNA having excitation max. at 651 nm, and emission max. at 670 nm
Kit content:
LDFO11000: 1mL (4*250µL) of LNP-DiO, (4.5 mM) encapsulated with 0.1 mg/ mL Cy5-mRNAFLuc having composition as described in table below:
| Lipid mix components | Molecular weight | Molar ratio | mM |
| FP105 | 731.2 | 50 | 2.29 |
|
DSPC |
790.17 | 9 | 0.41 |
| Cholesterol | 386.65 | 38.5 | 1.76 |
| DMG-PEG 2000 | 2509.2 | 1.5 | 0.07 |
| DiO | 881.7 | 1 | 0.05 |
| Total | 100 | 4.5 |
Quality Control
| Standard QC | Superior Grade QC* | |
| Size | ✔ | ✔ |
| Charge | ✔ | ✔ |
| Encapsulation efficiency | ✔ | ✔ |
| Sterility | ✔ | ✔ |
| RNA concentration | ✔ | ✔ |
| Fluorescent detection | ✔ | ✔ |
| Endotoxin | ✔ | |
| Mycoplasma detection | ✔ | |
| Lipid content | ✔ |
* Contact us to get a quote.
Storage
NanOZ Dual Fluo LNP-DiO_Cy5-mRNAFLuc must be stored at -80°C. We recommend to minimize freeze-thaw cycles to preserve LNPs integrity.
| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| LDFO10500 |
1ml
|
$1,929.00
|
|
| LDFO11000 |
0,5 ml
|
$1,157.00
|
|
| LDFO15000 |
5 ml
|
$7,716.00
|
|
LDFO10500
1ml
$1,929.00
LDFO11000
0,5 ml
$1,157.00
LDFO15000
5 ml
$7,716.00
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