Nanog mRNA
Nanog mRNAs have been designed to produce high expression level of Nanog protein.
OZB mRNAs are produced by in vitro transcription. mRNAs are stabilized with UTRs, a Cap1 structure at the 5’ end, and contain a poly(A) tail at the 3’ end.
Sequences have been optimized (codon optimization) to yield improved performance. Nanog mRNA #MRNA64 does not bear any additional nucleotide modifications while #MRNA65 is modified with 5-methoxyuridine (5moU), #MRNA66 is modified with N1-methyl-pseudouridine to reduce innate immune response.
Molecular weights:
- MRNA64: 367440 g/mol;
- MRNA65: 371670 g/mol;
- MRNA66: 369555 g/mol.
Quality Control:
| Items | Specification | Standard QC | Superior Grade QC* |
|---|---|---|---|
| Appearance | Clear | ✓ | ✓ |
| Integrity | Agarose gel mobility and fragment analyzer | ✓ | ✓ |
| Concentration | 1mg/ml +/- 5% | ✓ | ✓ |
| A260/280 |
>1.8 for Unmodified mRNAs >1.7 for chemically modified mRNAs |
✓ | ✓ |
| Sterility | Absence of bacterial growth at 37°C | ✓ | ✓ |
| Endotoxin | <0.5 EU/mL | ✓ | |
| dsRNA | <0.5% | ✓ | |
| Fonctionality | protein expression after transfection | ✓ |
* Our catalogue mRNAs undergo the standard QC. Superior Grade QC can be performed as an additional prestation. Contact us to get a quote.
mRNA length: 1136 nt.
Storage: mRNAs must be stored at -80°C.
We recommend to aliquot the mRNA solution for a better storage.
Kit contents:
- Nanog mRNAs-20: 20 μg of mRNA.
- Nanog mRNAs-100: 100 μg mRNA.
- Nanog mRNAs-1000: 1 mg of mRNA.
| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA64-20 |
Nanog mRNA unmodified - 20 µg
|
$183.00
|
|
| MRNA64-100 |
Nanog mRNA unmodified - 100 µg
|
$269.00
|
|
| MRNA64-1000 |
Nanog mRNA unmodified - 1mg
|
$1,472.00
|
|
| MRNA65-20 |
Nanog mRNA 5moU - 20µg
|
$183.00
|
|
| MRNA65-100 |
Nanog mRNA 5moU - 100µg
|
$343.00
|
|
| MRNA65-1000 |
Nanog mRNA 5moU - 1 mg
|
$1,792.00
|
|
| MRNA66-20 |
Nanog mRNA N1-mψ - 20 µg
|
$205.00
|
|
| MRNA66-100 |
Nanog mRNA N1-mψ - 100 µg
|
$365.00
|
|
| MRNA66-1000 |
Nanog mRNA N1-mψ - 1 mg
|
$1,813.00
|
|
MRNA64-20
Nanog mRNA unmodified - 20 µg
$183.00
MRNA64-100
Nanog mRNA unmodified - 100 µg
$269.00
MRNA64-1000
Nanog mRNA unmodified - 1mg
$1,472.00
MRNA65-20
Nanog mRNA 5moU - 20µg
$183.00
MRNA65-100
Nanog mRNA 5moU - 100µg
$343.00
MRNA65-1000
Nanog mRNA 5moU - 1 mg
$1,792.00
MRNA66-20
Nanog mRNA N1-mψ - 20 µg
$205.00
MRNA66-100
Nanog mRNA N1-mψ - 100 µg
$365.00
MRNA66-1000
Nanog mRNA N1-mψ - 1 mg
$1,813.00
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