F-Luc mRNA
F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
OZ Biosciences mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNAs contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance.
F-Luc mRNAs:
- #MRNA16 does not bear any additional nucleotide modifications.
- #MRNA12 is modified with 5-methoxyuridine (5moU) to reduce innate immune response.
- #MRNA24 is modified with N1-methyl-pseudouridine (N1-mψ) to reduce innate immune response.
- #MRNA12c and #MRNA16c are labelled with Cy5 (Sulfo-Cyanine5) by replacing 15% of U or moU by UTP-Cy5.
Quality Control
| Items | Specification | Standard QC | Superior Grade QC* |
|---|---|---|---|
| Appearance | Clear | ✓ | ✓ |
| Integrity | One peak, right size | ✓ | ✓ |
| One peak, right size | ✓ | ✓ | |
| Concentration | 1mg/ml +/- 5% | ✓ | ✓ |
| A260/280 | >1.8 for Unmod, >1.7 for modified | ✓ | ✓ |
| Sterility | Absence of growth after 14 days | ✓ | ✓ |
| Endotoxin | <0.5 EU/mL | ✓ | |
| dsRNA | <0.5% | ✓ | |
| Fonctionality | protein expression after transfection | ✓ | ✓ |
* Contact us to get a quote.
We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.
Size: 20 µg, 100µg and 1 mg
For bulk, please contact us: order@ozbiosciences.com
Storage: -80°C
Shipping : Dry ice (fee included in shipping cost)
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA16-20 |
F-Luc mRNA 20 µg
|
$176.00
|
|
| MRNA16-100 |
F-Luc mRNA 100 µg
|
$282.00
|
|
| MRNA16-1000 |
F-Luc mRNA 1mg
|
$1,556.00
|
|
| MRNA12-20 |
mRNA F-Luc (5moU) 20 µg
|
$184.00
|
|
| MRNA12-100 |
mRNA F-Luc (5moU) 100 µg
|
$350.00
|
|
| MRNA12-1000 |
mRNA F-Luc (5moU) 1mg
|
$1,878.00
|
|
| MRNA24-20 |
F-Luc mRNA (N1-mψ) 20 µg
|
$206.00
|
|
| MRNA24-100 |
F-Luc mRNA (N1-mψ) 100 µg
|
$372.00
|
|
| MRNA24-1000 |
F-Luc mRNA (N1-mψ) 1 mg
|
$1,901.00
|
|
MRNA16-20
F-Luc mRNA 20 µg
$176.00
MRNA16-100
F-Luc mRNA 100 µg
$282.00
MRNA16-1000
F-Luc mRNA 1mg
$1,556.00
MRNA12-20
mRNA F-Luc (5moU) 20 µg
$184.00
MRNA12-100
mRNA F-Luc (5moU) 100 µg
$350.00
MRNA12-1000
mRNA F-Luc (5moU) 1mg
$1,878.00
MRNA24-20
F-Luc mRNA (N1-mψ) 20 µg
$206.00
MRNA24-100
F-Luc mRNA (N1-mψ) 100 µg
$372.00
MRNA24-1000
F-Luc mRNA (N1-mψ) 1 mg
$1,901.00
Citation database
| NAME | MOLECULE VECTOR | PRODUCT | REFERENCE | |
| all cells | 0 | F-Luc mRNA | Hassanel DNBP. Int J Pharm. 2024 Sep 15:665:124695. | |
| all cells | 0 | F-Luc mRNA | Tai JA Vaccines (Basel). 2024 Jul 9;12(7):757. | |
| all cells | 0 | F-Luc mRNA | Kranse O. Thesis (U. Cambridge) 2024 | |
| all cells | 0 | F-Luc mRNA | Camperi J. Anal Chem. 2024 Feb 20. doi: 10.1021/acs.analchem.3c05539 | |
| all cells | 0 | F-Luc mRNA | Medjmedj A. Nanomaterials 2022 12(14) 2446 | |
| all cells | 0 | F-Luc mRNA | Kranse O. G3 (Bethesda). 2021 Feb 9;11(2):jkaa058 |
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