LIN28 mRNA
LIN28 mRNAs have been designed to produce high expression level of LIN28 protein.
OZB mRNAs are produced by in vitro transcription. mRNAs are stabilized at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end.
Sequences have been optimized to yield improved stability and performance. LIN28 mRNA #MRNA86 does not bear any additional nucleotide modifications while #MRNA87 is modified with 5-methoxyuridine (5moU), #MRNA88 is modified with N1-methyl-pseudouridine to reduce innate immune response.
Molecular weights:
- MRNA86: 275080 g/mol;
- MRNA87: 278320 g/mol;
- MRNA88: 276700 g/mol.
Quality Control:
| Items | Specification | Standard QC | Superior Grade QC* |
|---|---|---|---|
| Appearance | Clear | ✓ | ✓ |
| Integrity | Agarose gel mobility and fragment analyzer | ✓ | ✓ |
| Concentration | 1mg/ml +/- 5% | ✓ | ✓ |
| A260/280 |
>1.8 for Unmodified mRNAs >1.7 for chemically modified mRNAs |
✓ | ✓ |
| Sterility | Absence of bacterial growth at 37°C | ✓ | ✓ |
| Endotoxin | <0.5 EU/mL | ✓ | |
| dsRNA | <0.5% | ✓ | |
| Fonctionality | protein expression after transfection | ✓ |
* Our catalogue mRNAs undergo the standard QC. Superior Grade QC can be performed as an additional prestation. Contact us to get a quote.
mRNA length: 848 nt.
Storage: mRNAs must be stored at -80°C.
We recommend to aliquot the mRNA solution for a better storage.
Kit contents:
- LIN28 mRNAs-20: 20 μg of mRNA.
- LIN28 mRNAs-100: 100 μg mRNA.
- LIN28 mRNAs-1000: 1 mg of mRNA.
| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA86-20 |
Lin28 mRNA unmodified - 20 µg
|
$183.00
|
|
| MRNA86-100 |
Lin28 mRNA unmodified - 100 µg
|
$269.00
|
|
| MRNA86-1000 |
Lin28 mRNA unmodified - 1mg
|
$1,472.00
|
|
| MRNA87-20 |
Lin28 mRNA 5moU - 20µg
|
$183.00
|
|
| MRNA87-100 |
Lin28 mRNA 5moU - 100µg
|
$343.00
|
|
| MRNA87-1000 |
Lin28 mRNA 5moU - 1mg
|
$1,792.00
|
|
| MRNA88-20 |
Lin28 mRNA N1-mψ - 20 µg
|
$205.00
|
|
| MRNA88-100 |
Lin28 mRNA N1-mψ - 100 µg
|
$365.00
|
|
| MRNA88-1000 |
Lin28 mRNA N1-mψ - 1mg
|
$1,813.00
|
|
MRNA86-20
Lin28 mRNA unmodified - 20 µg
$183.00
MRNA86-100
Lin28 mRNA unmodified - 100 µg
$269.00
MRNA86-1000
Lin28 mRNA unmodified - 1mg
$1,472.00
MRNA87-20
Lin28 mRNA 5moU - 20µg
$183.00
MRNA87-100
Lin28 mRNA 5moU - 100µg
$343.00
MRNA87-1000
Lin28 mRNA 5moU - 1mg
$1,792.00
MRNA88-20
Lin28 mRNA N1-mψ - 20 µg
$205.00
MRNA88-100
Lin28 mRNA N1-mψ - 100 µg
$365.00
MRNA88-1000
Lin28 mRNA N1-mψ - 1mg
$1,813.00
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