LDH Cytotoxicity Assay Kit
The LDH Cytotoxicity Assay Kit is a colorimetric assay kit for the determination of cellular toxicity in vitro.
This assay kit measures the release of lactate dehydrogenase (LDH) from damaged and lysed cells in surrounding culture medium.
LDH is a stable cytosolic enzyme that allows the conversion of iodonitrotetrazolium (INT), a tetrazolium salt into formazan product in presence of an electron mediator. The amount of formazan produced is directly proportional to the release of LDH and thus, to lysed cells.
- No solubilisation process is required since this formazan does not require any solvation;
- Measurement can be performed directly in the tissue culture medium;
- More sensitive at neutral pH than other tetrazolium salts such as MTT, XTT, MTS or WST-1.
- Dedicated to measuring cell toxicity, late apoptosis or necrosis.
- Ready-to-use does not need cell fixation, cell lysis or washing steps
- Fast, Accurate, sensitive and adapted to high throughput screening
Sizes:
LDH Cytotoxicity Assay Kit 500 assays:
- 12.5mL of 2X INT Buffer (A)
- 12.5mL of 2X Assay Buffer (B)
- 100µL of 1000X Buffer (C)
- 25mL Stop Solution
- 1 mL Lysis Buffer (10X)
- 10 µL positive control
Storage: Upon reception, store the Lysis Buffer and Stop solution at 4°C and other components of the LDH cytotoxicity kit at -20°C. Protect from light and avoid freeze-thaw cycles.
Stability: 1 year at -20°C
Shipping Conditions: The kit is shipped at RT.
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| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| LDH0500 |
500 Assays
|
$368.00
|
|
Figure 1: Dosage of the activity of recombinant mammalian lactate dehydrogenase (LDH). Standard curve of LDH from 0 to 2.5u/mL was realized by serial two-fold dilutions in 50µL H2O in triplicate. 20min after addition of 50µL working solution, the absorbance was read at 490nm and 690nm to determine LDH activity.
Figure 2: LDH activity determined in HeLa cells: 24H after seeding, medium was removed and cells were lysed using the LDH lysis buffer (1X) and LDH activity was measured according to the protocol after 20 min incubation.
Figure 3: Cell toxicity confirmed by measuring LDH activity in KG1a cells after H2O2 stimulation (40min incubation time).Related products
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