Optimized and Stabilized mRNAs

OZ Biosciences offers mRNAs that mimic fully processed mature mRNAs. These mRNAs are stabilized with 5’ Cap 1 structure and 3’ poly(A) tail and are optimized to yield improved stability & performance. They are modified with 5-methoxyuridine (5moU replaces U) or N1-methyl-pseudouridine (N1-mψ) to reduce innate immune responses. We also offer unmodified mRNAs.

7 categories of ready-to-use mRNAs

• Reporter gene mRNAs: ideal controls to study transfection efficiency.
• Fluorescent mRNAs: Cy5 F-Luc mRNA, Cy5 GFP mRNA
• Genome editing mRNAs: ideal for Genome editing.
• Vaccine mRNAs: ideal as controls for immunization or vaccine studies.
• Gene Replacement mRNAs: EPO mRNA
• Interleukin mRNA: H IL-2 mRNA
• Transcription Factor mRNA: c-Myc mRNA, H Sox2 mRNA

Self-amplifying RNA (saRNA)

OZ Biosciences also offers saRNAs that are designed to produce high expression level of protein with enhanced length of duration. These saRNAs are stabilized with 5’ Cap 1 structure and 3’ poly(A) tail. They can be modified with 5-methyl-Cytidine (m5C) or unmodified.

• saRNA GFP
• saRNA LUC

Custom mRNA Synthesis Service

With over 15 years of nucleic acid delivery expertise, OZ Biosciences is confident in providing you with the best-in-class service for the production of high quality mRNAs.

We are able to synthesis custom mRNAs at microgram to multigram scales, from a few hundred up to several thousand bases, with a wide variety of modifications including 5’ terminal modifications with cap, internal modification such as 5-methoxyuridine (other modified nucleotides are available) and 3’ modification such as poly-A tail. Fluorescent labeling with Cy5, Cy3 or other options is available.

Production Process

The mRNA production process at OZ Biosciences has been meticulously perfected over the years to achieve an exceptional level of optimization. Using state-of-the-art enzymes, considered to be the most efficient available, the process guarantees production that minimizes dsRNA formation. This attention to detail results in mRNAs of the highest quality, reflecting an ongoing commitment to excellence and innovation in the field.

Quality control 

We do know that the reproducibility of scientific experiments is very important to researchers. That is why we developed a quality control system tailored to each product, enabling us to detect defective batches immediately.

Our mRNAs, whether custom or catalog, undergo standard quality control, including measurement of size, integrity and purity by fragment analyzer, agarose gel and Nanodrop (concentration 260/280 and 260/230), a sterility test and an experimental validation (transfection / expression). 

However, we have developed additional tests to extend the analysis to include endotoxin detection and double strand RNA (dsRNA) quantification.

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