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Product Launch Announcement: Self-amplifying RNA

BLOG > mRNA & LNP > Product Launch Announcement: Self-amplifying RNA

self-amplifying rna

We are pleased to announce the development of a new RNA category : saRNA (Self-amplifiying RNA). 

OZB saRNA have been constructed based on replicons of positive-sense ((+)-RNA) viruses (Venezuelan equine encephalitis Virus (VEEV)), where the coding sequence of the viral structural proteins is replaced with that of a gene of interest (GOI), while retaining the coding sequences of non-structural proteins (NsPs), including the viral RNA-dependent RNA polymerase. 

Please note that OZB saRNAs encodes also for Puromycin Resistance Cassette, which does not alter the expression of the GOI but can be used for cell selection.

saRNAs have been designed to produce high expression level of protein with enhanced length of duration. OZB mRNAs are produced by in vitro transcription. mRNAs are stabilized at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end. Sequences have been optimized to yield improved stability and performance.

Fig 1 : GFP protein expression over time in HEK293 cells in 24-well plate cells upon lipid-based transfection with either 0,4 µg/well of Replicon GFP or 0,4 µg/well of classic mRNA GFP using NL51 transfection reagent (ratio 2:1). Photos were taken at x20 show a peak of GFP expression at 24hic mRNA GFP.

More Results

Discover our saRNAs range:

saRNA GFP

saRNA GFP, designed to produce high expression level of GFP protein with enhanced length of duration.

Available in two ways:

  • #MRNA70 does not bear any additional nucleotide modifications.
  • #MRNA71 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).
saRNA GFP
saRNA Luc

saRNA Luc, designed to produce high expression level of Luciferase protein with enhanced length of duration.

Available in two ways:

  • #MRNA72 does not bear any additional nucleotide modifications.
  • #MRNA73 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).
saRNA Luc
saRNA OVA

saRNA OVA, designed to produce high expression level of Ova protein with enhanced length of duration.

Available in two ways:

  • #MRNA74 does not bear any additional nucleotide modifications.
  • #MRNA75 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).
saRNA Ova
saRNA HA(FL)-H1N1

saRNA HA(FL)-H1N1, designed to produce high expression level of HA(FL)-H1N1 protein with enhanced length of duration.

Available in two ways:

  • #MRNA76 does not bear any additional nucleotide modifications.
  • #MRNA77 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).
saRNA HA(FL)-H1N1
saRNA Spike SARS CoV-2

saRNA Spike SARS CoV-2, designed to produce high expression level of SPIKE SARS COV-2 (E484K; N501Y) protein with enhanced length of duration.

Available in two ways:

  • #MRNA78 does not bear any additional nucleotide modifications.
  • #MRNA79 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).
saRNA Spike SARS CoV-2

Catalog saRNAsCustom saRNA

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