GFP saRNA
Ready-to-use stabilized GFP saRNA
GFP self-amplifying RNAs have been designed to produce high expression level of GFP protein with enhanced length of duration (see results). saRNAs are produced by in vitro transcription. saRNAs are stabilized at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end. Sequences have been optimized to yield improved stability and performance.
GFP saRNAs are also available capped with ExploCap™.
OZ Biosciences GFP saRNA has been constructed based on replicons of positive-sense ((+)-RNA) viruses (Venezuelan equine encephalitis Virus (VEEV)), where the coding sequence of the viral structural proteins is replaced with that of a gene of interest (GOI, here GFP), while retaining the coding sequences of non-structural proteins (NsPs), including the viral RNA-dependent RNA polymerase. Please note that OZB saRNAs encodes also for Puromycin Resistance Cassette, which does not alter the expression of the GOI but can be used for cell selection.
- #MRNA70 does not bear any additional nucleotide modifications.
- #MRNA71 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).
Concentration: 1.0 mg/mL in 1 mM Sodium Citrate, pH 6.4
GFP mRNA length: 9821nt
Size: 20µg, 100µg and 1mg
Storage: saRNAs must be stored at -80°C. We recommend to aliquot the RNA solution for a better storage and avoid freeze/thaw cycles.
For Bulk, please contact us : order@ozbiosciences.com
| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA70-20 |
GFP saRNA 20µg (unmodified)
|
$120.00
|
|
| MRNA70-100 |
GFP saRNA 100µg (unmodified)
|
$375.00
|
|
| MRNA70-1000 |
GFP saRNA 1mg (unmodified)
|
$1,975.00
|
|
| MRNA71-20 |
GFP saRNA 20µg (m5C)
|
$170.00
|
|
| MRNA71-100 |
GFP saRNA 100µg (m5C)
|
$400.00
|
|
| MRNA71-1000 |
GFP saRNA 1mg (m5C)
|
$2,075.00
|
|
Citation database
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