saRNA GFP
OZB saGFP RNA has been constructed based on replicons of positive-sense ((+)-RNA) viruses (Venezuelan equine encephalitis Virus (VEEV)), where the coding sequence of the viral structural proteins is replaced with that of a gene of interest (GOI, here GFP), while retaining the coding sequences of non-structural proteins (NsPs), including the viral RNA-dependent RNA polymerase. Please note that OZB saRNAs encodes also for Puromycin Resistance Cassette, which does not alter the expression of the GOI but can be used for cell selection.
SaGFP RNAs have been designed to produce high expression level of GFP protein with enhanced length of duration (see results). OZB mRNAs are produced by in vitro transcription. mRNAs are stabilized at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end. Sequences have been optimized to yield improved stability and performance.
- #MRNA70 does not bear any additional nucleotide modifications.
- #MRNA71 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).
Quality Control
| Standard QC | Superior Grade QC* | |
| Fragment analyzer | ✔ | ✔ |
| Agarose gel | ✔ | ✔ |
| Concentration (260/280 & 260/230) | ✔ | ✔ |
| Sterility test | ✔ | ✔ |
| Transfection/Expression | ✔ | ✔ |
| Endotoxin | ✔ | |
| dsRNA | ✔ |
* Contact us to get a quote.
Ready-to-use stabilized saGFP puroR RNA
Concentration: 1.0 mg/mL in 1 mM Sodium Citrate, pH 6.4
mRNA length: 9821nt .
#MRNA70 Molecular weight: 3,17 E+06 g/mol.
#MRNA71 Molecular weight: 3,20 E+06 g/mol.
Size: 20µg, 100µg and 1mg
For Bulk, please contact us : order@ozbiosciences.com
Storage
RNAs must be stored at -80°C.
We recommend to aliquot the RNA solution for a better storage and avoid freeze/thaw cycles.
| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA70-20 |
saRNA GFP 20µg (unmodified)
|
$320.00
|
|
| MRNA70-100 |
saRNA GFP 100µg (unmodified)
|
$700.00
|
|
| MRNA70-1000 |
saRNA GFP 1mg (unmodified)
|
$3,750.00
|
|
| MRNA71-20 |
saRNA GFP 20µg (m5C)
|
$420.00
|
|
| MRNA71-100 |
saRNA GFP 100µg (m5C)
|
$900.00
|
|
| MRNA71-1000 |
saRNA GFP 1mg (m5C)
|
$4,500.00
|
|
MRNA70-20
saRNA GFP 20µg (unmodified)
$320.00
MRNA70-100
saRNA GFP 100µg (unmodified)
$700.00
MRNA70-1000
saRNA GFP 1mg (unmodified)
$3,750.00
MRNA71-20
saRNA GFP 20µg (m5C)
$420.00
MRNA71-100
saRNA GFP 100µg (m5C)
$900.00
MRNA71-1000
saRNA GFP 1mg (m5C)
$4,500.00
Citation database
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