saRNA Luc
OZB saLuc RNA has been constructed based on replicons of positive-sense ((+)-RNA) viruses (Venezuelan equine encephalitis Virus (VEEV)), where the coding sequence of the viral structural proteins is replaced with that of a gene of interest (GOI, here LUC), while retaining the coding sequences of non-structural proteins (NsPs), including the viral RNA-dependent RNA polymerase. Please note that OZB saRNAs encodes also for Puromycin Resistance Cassette, which does not alter the expression of the GOI but can be used for cell selection.
SaGFP RNAs have been designed to produce high expression level of Luciferase protein with enhanced length of duration (see results). OZB mRNAs are produced by in vitro transcription. mRNAs are stabilized at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end. Sequences have been optimized to yield improved stability and performance.
- #MRNA72 does not bear any additional nucleotide modifications.
- #MRNA73 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).
Quality Control
| Standard QC | Superior Grade QC* | |
| Fragment analyzer | ✔ | ✔ |
| Agarose gel | ✔ | ✔ |
| Concentration (260/280 & 260/230) | ✔ | ✔ |
| Sterility test | ✔ | ✔ |
| Transfection/Expression | ✔ | ✔ |
| Endotoxin | ✔ | |
| dsRNA | ✔ |
* Contact us to get a quote.
Ready-to-use stabilized saLUC puroR RNA
Concentration: 1.0 mg/mL in 1 mM Sodium Citrate, pH 6.4
mRNA length: 10741 nt .
#MRNA72 Molecular weight: 3,46 E+06 g/mol.
#MRNA73 Molecular weight: 3,50 E+06 g/mol.
Size: 20µg, 100µg and 1mg
For Bulk, please contact us : order@ozbiosciences.com
Storage
RNAs must be stored at -80°C.
We recommend to aliquot the RNA solution for a better storage and avoid freeze/thaw cycles.
| Catalog number | Unit Size | Price excl tax | Quantity / Buy |
| MRNA72-20 |
saRNA LUC 20µg (unmodified)
|
$320.00
|
|
| MRNA72-100 |
saRNA LUC 100µg (unmodified)
|
$700.00
|
|
| MRNA73-20 |
saRNA LUC 20µg (m5C)
|
$420.00
|
|
| MRNA72-1000 |
saRNA LUC 1mg (unmodified)
|
$3,750.00
|
|
| MRNA73-100 |
saRNA LUC 100µg (m5C)
|
$900.00
|
|
| MRNA73-1000 |
saRNA LUC 1mg (m5C)
|
$4,500.00
|
|
MRNA72-20
saRNA LUC 20µg (unmodified)
$320.00
MRNA72-100
saRNA LUC 100µg (unmodified)
$700.00
MRNA73-20
saRNA LUC 20µg (m5C)
$420.00
MRNA72-1000
saRNA LUC 1mg (unmodified)
$3,750.00
MRNA73-100
saRNA LUC 100µg (m5C)
$900.00
MRNA73-1000
saRNA LUC 1mg (m5C)
$4,500.00
Citation database
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