BLOG > Publications & Citations > Activity-dependent 5′tRF GluCTC modulates neuronal networks in hiPSC neurons
Authors: Stewart, R., Zaheer, S., Düssmann, H. et al.
Source: Cell Death Dis (2026).
We're delighted to share insights from a recent study entitled "Activity-dependent release of 5′tRF GluCTC modulates neuronal molecular networks in a human induced pluripotent stem cell-derived neuronal model" published in Cell Death & Disease by Rachel Stewart et al.
They investigated the activity-dependent generation and extracellular release of 5′tRF GluCTC in hiPSC-derived cortical neurons. Pharmacological induction of neuronal hyperexcitability increased both intracellular and extracellular levels of 5′tRF GluCTC. Exogenous delivery of a 5′tRF GluCTC mimic induced broad proteomic remodeling, predominantly downregulating proteins involved in RNA-protein complexes, translation, and neuronal signaling, with several affected proteins associated with epilepsy and other neurological disorders. These findings identify 5′tRF GluCTC as an activity-dependent, stress-responsive regulator of neuronal proteostasis, linking hyperexcitability to translational dysregulation and altered network activity.
Congratulations to all authors for this great article.
NeuroMag was used to transfect cortical neurons at DIV 80 with a 6FAM-labeled 5′tRF GluCTC mimic and scrambled control for RNA, protein, and proteomic analyses by mass spectrometry.
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